Fungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast.

Several different transformation techniques have been developed over the years and readily shown to be decisive methodsÂ{u9BA0}fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examplesÂ{u5CE5}d in laboratories worldwide. Not only will this text provide a detailedÂ{u2863}kground onÂ{u1C30}lications inÂ{u9BA4}ustrial and pharmaceutical relevant microbes, but also theÂ{u9B70}ortance of fungal pathogensÂ{u9BA0}agricultural production (Phytophthora and Botrytis)Â{u1BA4} mammalian infection (Penicillium marneffei and Candida).Â{u796E}netic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungalÂ{u4CA1}its.