Genetic Transformation Systems in Fungi Volume 1
Genetic Transformation Systems in Fungi Volume 1
edited by Marco A. van den Berg, Karunakaran Maruthachalam.
- 1 recurso en línea (XV, 318 p.) 69 ilustraciones, 17 ilustraciones en color
- Fungal Biology 2198-7777 .
- Fungal Biology .
Fungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast.
Several different transformation techniques have been developed over the years and readily shown to be decisive methodsÂfungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examplesÂd in laboratories worldwide. Not only will this text provide a detailedÂkground onÂlications inÂustrial and pharmaceutical relevant microbes, but also theÂortance of fungal pathogensÂagricultural production (Phytophthora and Botrytis)Â mammalian infection (Penicillium marneffei and Candida).Ânetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungalÂits.
9783319101422
10.1007/978-3-319-10142-2 doi
Hongos
QK602 / 2015 EB
Fungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast.
Several different transformation techniques have been developed over the years and readily shown to be decisive methodsÂfungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examplesÂd in laboratories worldwide. Not only will this text provide a detailedÂkground onÂlications inÂustrial and pharmaceutical relevant microbes, but also theÂortance of fungal pathogensÂagricultural production (Phytophthora and Botrytis)Â mammalian infection (Penicillium marneffei and Candida).Ânetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungalÂits.
9783319101422
10.1007/978-3-319-10142-2 doi
Hongos
QK602 / 2015 EB