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999			_c96647 _d96647 _x1
001			96647
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005			20230102112804.0
006			m o d
007			cr cnu|||unuuu
008			171101s2017 sz a o 000 0 eng d
020			_a3319673580 _q(electronic bk.)
020			_a9783319673585 _q(electronic bk.)
020			_z3319673572
020			_z9783319673578 _q(print)
040			_aN$T _beng _erda _epn _cN$T _dGW5XE _dN$T _dYDX _dUAB _dEBLCP _dIOG _dCOO _dAZU _dUPM _dVT2 _dOCLCF _dCASUM _dES-MaUEC _bspa
050		4	_aR857.T55 _b2017 EB
245	0	0	_aMulti-parametric live cell microscopy of 3D tissue models _cRuslan I. Dmitriev, editor.
264		1	_aCham, Switzerland _bSpringer International Publishing _c2017
300			_a1 recurso en línea _bilustraciones (algunas a color)
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_atext file _bPDF
490	0		_aAdvances in experimental medicine and biology _x0065-2598
500			_a
505	0		_a""Preface""; ""Contents""; ""Contributors""; ""Part I: Introduction: 3D Tissue Models, Methodology and Toolkit""; ""1: Current State-of-the-Art 3D Tissue Models and Their Compatibility with Live Cell Imaging""; ""1.1 Introduction""; ""1.2 Types of 3D Tissue Models""; ""1.2.1 Scaffold-Free Cultures""; ""1.2.2 Scaffold-Cell Constructs""; ""1.3 Imaging Modalities for Live Cells""; ""1.3.1 Confocal Laser Scanning Microscopy and Epifluorescence Microscopy""; ""1.3.2 Fluorescence Lifetime Imaging (FLIM) and Phosphorescence Lifetime Imaging (PLIM)""
505	8		_a""1.3.3 Optical Coherence Tomography""""1.3.4 Micro-Computerised Tomography""; ""1.3.5 Indirect Live Cell Imaging""; ""1.3.5.1 Monitoring Scaffold Degradation""; ""1.3.5.2 Monitoring Extracellular Matrix Deposition by Live Cells""; ""1.4 Advantages and Challenges of Live Cell Imaging""; ""1.5 Prospect and Summary""; ""References""; ""2: Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation""; ""2.1 Motivation of Using Phosphorescence Lifetime Imaging""; ""2.2 Technical Challenges""
505	8		_a""2.2.1 Excitation Pulse Period and Laser Power""""2.2.2 Pile-Up Effect""; ""2.2.3 Detector Overload""; ""2.2.4 Interference with Scanning""; ""2.3 FLIMâ#x80;#x94;PLIM by Multipulse Excitation""; ""2.4 Implementation in Laser Scanning Systems""; ""2.4.1 DCS-120 Confocal Scanning FLIM System""; ""2.4.2 Zeiss LSM 710, 780, 880 Systems""; ""2.4.3 Leica SP Multiphoton Systems""; ""2.4.4 Sutter Instrument MOM Microscopes""; ""2.5 Applications""; ""2.5.1 Oxygen Sensing""; ""2.5.2 Simultaneous Recording of pO2 and NAD(P)H Images""; ""2.5.3 Detection of Zinc Oxide Nanoparticles""
505	8		_a""2.5.4 PLIM of Inorganic Materials""""2.6 Suppression of Autofluorescence""; ""2.7 Summary""; ""References""; ""3: Quantitative Live Cell FLIM Imaging in Three Dimensions""; ""3.1 Introduction""; ""3.2 Implementations of Fluorescence Lifetime Imaging""; ""3.2.1 Time-Domain FLIM Systems""; ""3.2.1.1 Time-Correlated Single Photon Counting (TCSPC)""; ""Confocal TCSPC-FLIM""; ""Multi-Photon TCSPC-FLIM""; ""3.2.1.2 Time-Gated systems""; ""3.2.2 Frequency Domain systems""; ""3.3 Multi-Channel FLIM""; ""3.3.1 Multi-Color and Spectrally-­Resolved FLIM""
505	8		_a""3.3.2 Polarization-Resolved FLIM""""3.4 FLIM in Combination with Other Microscopy Techniques""; ""3.4.1 Combined FLIM and PLIM""; ""3.4.2 FLIM and Optical Coherence Tomography (OCT)""; ""3.4.3 FLIM with Nonlinear Microscopy Techniques for Label-Free Imaging of Complex Tissue""; ""3.4.4 FLIM for Clinical Imaging""; ""3.4.5 Perspective: Adaptive Optics for Improved Optical Sectioning in High Resolution Microscopy""; ""3.5 Analysis of FLIM Data""; ""3.5.1 Time-Domain Data""; ""3.5.1.1 Pixel-Wise Decay Fitting""; ""3.5.1.2 Global Fitting Techniques""
520	3		_aThis book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
988			_aEBOOK, asignarmaterias, EBSPRINGER_2017
650		7	_9143820 _aIngeniería biomédica _2fast _0(OCoLC)fst00832568 _0
700	1		_aDmitriev, Ruslan I., _eeditor literario
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=http://link.springer.com/10.1007/978-3-319-67358-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2018 _dz _e- _zSI