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020 _a9789811045103
020 _a9789811045110
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050 4 _aRB43.7
_b2017 EB
245 0 0 _aMolecular diagnostics.
_nPart 2,
_pClinical, veterinary, agrobotanical and food safety applications
_cedited by E. van Pelt-Verkuil, W.B. van Leeuwen, R. te Witt.
246 3 0 _aClinical, veterinary, agrobotanical and food safety applications
264 1 _aSingapore
_bSpringer
_c2017.
300 _a1 recurso en línea (vii, 237 páginas)
_bilustraciones (algunas a color)
336 _aTexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _atext file
_bPDF
500 _aIncluye índice
500 _aSpringerLink
_bSpringer Biomedical and Life Sciences eBooks 2017 English+International
505 0 _aIntroduction; 1 Molecular Diagnostics of Bacterial Pathogens; Abstract; 1.1 Introduction; 1.2 Molecular Diagnostics of Bacterial Sexually Transmitted Infections; 1.2.1 Molecular Diagnostics of Chlamydia trachomatis and Neisseria gonorrhoeae; 1.2.2 Molecular Diagnostics of Other Sexually Transmitted Bacteria; 1.3 Molecular Diagnostics of Bacterial Gastroenteritis; 1.3.1 Major Bacterial Pathogens for Routine Microbiological Diagnostics; 1.3.2 Other Bacterial Pathogens; 1.3.3 Molecular Diagnostics Algorithms for the Identification of Enteric Pathogens; 1.4 Bacterial Typing.
505 8 _a1.4.1 Typing Methods1.4.2 Pulsed-Field Gel Electrophoresis (PFGE); 1.4.3 Amplified Fragment Length Polymorphism Typing (AFLP); 1.4.4 Variable Number of Tandem Repeat (VNTR) Typing; 1.4.5 Random Amplification of Polymorphic DNA (RAPD); 1.4.6 Other Amplification Based Methods; 1.4.7 Single Locus Sequence Typing; 1.4.8 Spa Typing; 1.4.9 Multilocus Sequence Typing; 1.4.10 Optical Mapping; 1.4.11 Whole Genome Sequencing (WGS); 1.4.12 Typing for Action; 1.5 Conclusion; Recommended Literature; 2 Molecular Diagnostics of Parasitic Infections; Abstract; 2.1 Introduction; 2.2 DNA Isolation.
505 8 _a2.3 Intestinal Parasites2.3.1 Protozoa; 2.3.2 Helminths (Worms); 2.3.3 Algorithm for Faecal Examination; 2.4 Blood and Tissue Parasites; 2.4.1 Protozoa; 2.4.2 Helminths; 2.5 Concluding Remarks; References and Recommended Literature; 3 Virology; Abstract; 3.1 Introduction; 3.2 Isolation of Viral Nucleic Acids from Clinical Samples; 3.3 Molecular Diagnostics of Viruses; 3.3.1 Viral Detection Methods; 3.3.2 Primer/Probe Design; 3.3.2.1 Mismatch-Tolerance in Primers; 3.3.2.2 Probe Design; 3.4 Molecular Techniques for the Detection of Viral Variation.
505 8 _a3.5 Clinical Applications of Molecular Diagnostics3.5.1 Molecular Detection of Viral Infections; 3.5.2 Quantitative Detection of Viruses-Viral Load; 3.5.3 Genotyping and Genotypic Determination of Viral Resistance; 3.5.3.1 HIV-1; 3.5.3.2 Hepatitis B; 3.5.3.3 Hepatitis C; 3.5.3.4 Herpes Viruses; 3.5.3.5 Influenza Virus; 3.6 Conclusions; Recommended Literature; 4 Mycology; Abstract; 4.1 Introduction; 4.2 Molecular detection of invasive fungal infections; 4.2.1 Invasive Fungal Infections; 4.2.1.1 Diagnosis of Invasive Fungal Infection; 4.2.1.2 Conventional Species Identification.
505 8 _a4.2.1.3 Molecular Detection Methods4.2.1.4 Choice of Specimen; 4.2.1.5 Isolation of Fungal DNA; 4.2.2 Invasive Candidiasis; 4.2.2.1 Molecular Detection of Invasive Candidiasis; 4.2.3 Invasive Aspergillosis; 4.2.3.1 Molecular Detection; 4.2.4 Mucorales, Scedosporium, Fusarium and Other Opportunistic Filamentous Moulds; 4.2.4.1 Molecular Detection; 4.2.5 Pneumocystis jiroveci Pneumonia; 4.2.5.1 Molecular Identification; 4.3 Molecular diagnostics of dermatophyte infections; 4.3.1 Introduction; 4.3.2 Dermatophytosis; 4.3.3 Taxonomy and Epidemiology of Dermatophytes.
520 3 _aThis two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine Plant Pathology Food safety The two volumes are written by experts in their respective fields, who have, together with the editors, combined years of experience in the development, application and quality control of molecular diagnostic methods. The first book is devoted to the theory and backgrounds of molecular techniques, amplification technology, next generation sequencing and bioinformatics for molecular laboratory diagnostics. As a fundament of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is extensively discussed. The second book highlights the applications of these methods in the various diagnostic laboratories. These two full-colour well-illustrated volumes are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic tests and for those who wish to expand their knowledge on the current molecular biological revolution. The extensive information in both books highlights the current trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.
650 7 _aDiagnóstico molecular
_2embne
_0(OCoLC)fst01024777
_0
_9158916
700 1 _aLeeuwen, W. B. van,
_eautor
700 1 _aPelt-Verkuil, Elizabeth van,
_d1951-
_eautor
700 1 _aWitt, R. te,
_eautor
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=http://link.springer.com/10.1007/978-981-10-4511-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
988 _aEBOOK, asignarmaterias, EBSPRINGER_2017D
998 _b02/2018
_dz
_e-
_zSI
999 _c96348
_d96348
_x1