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008			170502s2017 sz a o 001 0 eng d
020			_a3319526731 _q(electronic bk.)
020			_a9783319526737 _q(electronic bk.)
020			_z3319526715
020			_z9783319526713 _q(print)
040			_aN$T _cN$T _dGW5XE _dEBLCP _dYDX _dN$T _dAZU _dUPM _dOCLCF _dCOO _dUAB _dESU _dIOG _dMERER _dVT2 _dOCLCQ _dU3W _dES-MaUEC _bspa
050		4	_aQP625.N89 _bN663 2017 EB
245	0	0	_aNon-canonical cyclic nucleotides _cRoland Seifert, editor.
264		1	_aCham, Switzerland _bSpringer _c2017.
300			_a1 recurso en línea (viii, 387 páginas) _bilustraciones (algunas a color)
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	0		_aHandbook of experimental pharmacology _x0171-2004 _vvolume 238
500			_aIncluye índice
500			_aSpringerLink _bSpringer Biomedical and Life Sciences eBooks 2017 English+International
505	0		_aPreface; Contents; cCMP and cUMP Across the Tree of Life: From cCMP and cUMP Generators to cCMP- and cUMP-Regulated Cell Functions; 1 Introduction; 2 Overview of cAMP and cGMP Signaling; 3 cCMP and cUMP Generators; 3.1 Initial Problems and Revitalization of the Field; 3.2 Soluble Guanylyl Cyclase; 3.3 Soluble Adenylyl Cyclase; 4 Occurrence of cCMP and cUMP in Biological Systems; 5 cCMP- and cUMP-Regulated Cell Functions; 6 Conclusions and Future Perspectives; References; cCMP and cUMP in Apoptosis: Concepts and Methods; 1 Introduction.
505	8		_a2 Cyclic Nucleotides and Induction of Apoptotic Pathways via Membrane-Permeant cNMP-Analogs3 Function of PKA, cAMP, cGMP, and cUMP in Apoptosis; 3.1 PKA in Apoptosis; 3.2 cAMP in Apoptosis; 3.3 cGMP in Apoptosis; 4 Hitherto Known Targets of cCMP and cUMP; 5 cCMP in Cell Proliferation and Apoptosis; 5.1 Relevant Genes in Apoptosis; 5.2 Cell Cycle Analysis: G1 Arrest; 5.3 Interaction of cCMP and cUMP with Bcl-2 Family Proteins and Induction of Apoptosis; 6 Experimental Methods; 6.1 Determination of Cell Number and Cell Proliferation; 6.1.1 Cell Number: Cell Counting After Trypan Blue Staining.
505	8		_a3 Nucleotide Binding Sites4 Promiscuity for Nucleobases; References; The Pseudomonas aeruginosa Exoenzyme Y: A Promiscuous Nucleotidyl Cyclase Edema Factor and Virulence Determinant; 1 Introduction; 2 The Endothelial cAMP Signal as a Barrier Protective Mechanism; 3 Edema Factor: A Soluble Adenylyl Cyclase; 4 Studies on the Discovery of ExoY and Its Enzymatic Activity; 5 ExoY and Disruption of the Endothelial Cytoskeleton; 6 Discovery That ExoY Is a Guanylyl Cyclase; 7 ExoY as a Purine and Pyrimidine Cyclase.
505	8		_a6.1.2 Cell Proliferation: BrdU Incorporation6.1.3 Thymidine Incorporation; 6.1.4 TUNEL Assay; 6.2 Detection of Apoptosis Using Flow Cytometry; 6.2.1 Annexin V/Propidium Iodide Staining; 6.2.2 Intracellular Staining with Cytochrome c-FITC-Antibody; 6.2.3 Cell Cycle Analysis; 6.3 Determination of the Mitochondrial Membrane Potential; 6.4 Detection of Caspases by Western Blotting and Fluorometric Assays; 7 Conclusion and Further Perspectives; References; Mammalian Nucleotidyl Cyclases and Their Nucleotide Binding Sites; 1 Introduction; 2 Overall Structure of Catalytic Domains.
505	8		_a8 The Intracellular Function of Purine and Pyrimidine Cyclic Nucleotide Monophosphates9 Is ExoY Relevant to Disease?; 10 Evidence That ExoY Does Not Contribute to Virulence; 11 Evidence That ExoY Contributes to Virulence; 12 Summary; References; Cyclic Nucleotide Monophosphates in Plants and Plant Signaling; 1 A Brief History of Cyclic Mononucleotide Signaling in Plants; 2 Evidence for Noncanonical Cyclic Nucleotide Monophosphates in Plants; 3 Cyclic Mononucleotides and Their Role in Plant Reactive Oxygen Species (ROS) Signaling.
520	3		_aThe cyclic purine nucleotides 3',5'-cAMP and 3',5'-cGMP are well-established second messengers. cGMP has recently been covered in a volume of the Handbook of Experimental Pharmacology (volume 191). In addition to 3',5'-cAMP and 3',5'-cGMP, so-called non-canonical cyclic nucleotides exist. These comprise the cyclic pyrimidine nucleotides 3',5'-cCMP and 3',5'-cUMP, the purine nucleotide 3',5'-cIMP, the 2',3'-nucleoside monophosphates and cyclic dinucleotides. In this volume of the Handbook of Pharmacology, word-leading experts in the field summarize our current knowledge on these non-canonical cyclic nucleotides, discuss open questions, future research directions and the pharmacotherapeutic implications. Special emphasis will be given to the emerging roles of 3',5'-cCMP and 3',5'-cUMP as second messengers with regard to generators, effectors, biological functions, inactivation and bacterial toxins. The role of 3',5'-cIMP as potential second messenger will also be critically discussed. Furthermore, we will consider transport of cyclic nucleotides and their potential role as first messengers. The role of the cyclic dinucleotide cGAMP in the immune system will covered, too. Lastly, the book will present important methodological aspects ranging from mass-spectrometric methods for cyclic nucleotide detection to the synthesis of nucleotide analogs as experimental tools and holistic methods for analysis of cyclic nucleotide effects.
650		7	_aNucleótidos _2embne _0(OCoLC)fst00885848 _0 _9144345
700	1		_aSeifert, Roland, _eeditor literario _9671720
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=http://link.springer.com/10.1007/978-3-319-52673-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
988			_aEBOOK, asignarmaterias, EBSPRINGER_2017C
998			_b02/2018 _dz _e- _zSI
999			_c95912 _d95912 _x1