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| 008 | 170426s2017 sz a o 001 0 eng d | ||
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_a3319491970 _q(electronic bk.) |
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_aQR65 _b.M634 2017 EB |
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| 245 | 0 | 0 |
_aModern tools and techniques to understand microbes _cAjit Varma, Arun Kumar Sharma, editors. |
| 264 | 1 |
_aCham, Switzerland _bSpringer _c2017. |
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| 300 |
_a1 recurso en línea (xiii, 461 páginas) _bilustraciones (algunas a color) |
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| 336 |
_aTexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 500 | _aIncluye índice | ||
| 500 |
_aSpringerLink _bSpringer Biomedical and Life Sciences eBooks 2017 English+International |
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| 505 | 0 | _aForeword; Preface; Contents; Chapter 1: Introduction to Modern Tools and Techniques to Understand Microbes; 1.1 Introduction; 1.2 Motives for Studying Microbial Diversity; 1.2.1 Factors Affecting Microbial Diversity (Zhao et al. 2012); 1.2.1.1 Abiotic Factors; 1.2.1.2 Biotic Factors; 1.3 Tools and Techniques to Understand Microbes; 1.4 Traditional Methods; 1.4.1 Total Microbial Plate Counts; 1.4.2 Sole-Carbon-Source Utilization; 1.4.3 Phospholipid Fatty Acid Analysis; 1.4.4 Light Microscopy; 1.4.4.1 Bright Field Microscope; 1.4.4.2 Dark Field Microscope; 1.4.4.3 Fluorescence Microscope. | |
| 505 | 8 | _a1.4.4.4 Phase Contrast Microscope1.4.4.5 Differential Interference Contrast Microscope; 1.4.4.6 Confocal Microscope; 1.4.5 Electron Microscopy; 1.4.5.1 Transmission Electron Microscope; 1.4.5.2 Scanning Electron Microscope; 1.4.5.3 Scanning Probe Microscopy; 1.5 Identification of Microbes; 1.5.1 Simple Staining; 1.5.2 Differential Staining; 1.5.3 Gram Staining; 1.5.4 Acid-Fast Staining; 1.5.5 Endospore Staining; 1.5.6 Negative Staining; 1.5.7 Flagella Staining; 1.6 Modern Molecular Methods; 1.6.1 Polymerase Chain Reaction; 1.6.2 Mole% G+C; 1.6.3 DNA Reassociation. | |
| 505 | 8 | _a1.6.4 Nucleic Acid Hybridization1.6.5 Restriction Fragment Length Polymorphism; 1.6.6 Terminal Restriction Fragment Length Polymorphism; 1.6.7 Ribosomal Intergenic Spacer Analysis/Automated Ribosomal Intergenic Spacer Analysis/Amplified Ribosomal DNA Restriction ... ; 1.7 DNA Microarrays; 1.8 Denaturant Gradient Gel Electrophoresis/Temperature Gradient Gel Electrophoresis; 1.9 Single Strand Conformation Polymorphism; 1.10 Next-Generation Sequencing; 1.11 Metagenomics; References; Chapter 2: Novel Approaches to Identify and Characterise Microorganisms in Food Industry; 2.1 Introduction. | |
| 505 | 8 | _a2.2 Molecular Approaches to Detect and Identify Food-Borne Microorganisms2.2.1 Nucleic Acid-Based Methods; 2.2.1.1 Nucleic Acid Hybridisation; 2.2.1.2 Polymerase Chain Reaction; RAPD-PCR; RTi-PCR; MPCR; PCR-RFLP; DNA Sequencing; MLST; 2.2.1.3 Pulsed-Field Gel Electrophoresis; 2.2.1.4 Ribotyping; 2.2.2 Proteomics; 2.3 Conclusions; References; Chapter 3: Strategies of Mass Cultivation of Microbes: Sebacinales; 3.1 Introduction; 3.2 Morphology and Taxonomy of Sebacinales; 3.3 Morphogenesis of P. indica; 3.4 Nutrient Requirements for Growth; 3.5 Media Composition. | |
| 505 | 8 | _a3.6 Growth in Batch Culture on Shaker3.6.1 Preparation of Media; 3.6.2 Sterilisation of Medium; 3.6.3 Inoculum Preparation; 3.6.4 Growth in Flasks on Shaker; 3.6.5 Harvesting of Biomass; 3.7 Production of Fungal Cultures in Fermenters; 3.7.1 Medium for Optimal Growth; 3.7.2 Sterilisation of the Fermenter; 3.7.3 Cultivation in Fermenter; 3.7.4 Recovery of Biomass Produced; 3.8 Production of Nanomycorrhiza; 3.8.1 Synthesis of Nanoparticles; 3.8.2 Preparation of Nanomycorrhiza; 3.8.3 Growth of Nanomycorrhiza; 3.9 Conclusions; References. | |
| 520 | 3 | _aThis book provides essential molecular techniques and protocols for analyzing microbes that are useful for developing novel bio-chemicals, such as medicines, biofuels, and plant protection substances. The topics and techniques covered include: microbial diversity and composition; microorganisms in the food industry; mass cultivation of sebacinales; host-microbe interaction; targeted gene disruption; function-based metagenomics to reveal the rhizosphere microbiome; mycotoxin biosynthetic pathways; legume-rhizobium symbioses; multidrug transporters of yeast; drug-resistant bacteria; the fungal endophyte piriformospora indica; medicinal plants; arbuscular mycorrhizal fungi; biosurfactants in microbial enhanced oil recovery; and biocontrol of the soybean cyst nematode with root endophytic fungi; as well as microbe-mediated drought tolerance in plants." Demonstrates modern laboratory procedures for analyzing microbes " Provides up-to-date techniques for research and teaching " Offers novel approaches to identify and characterize microorganisms. | |
| 650 | 7 |
_aMicrobiología _xTechnique. _2embne _0(OCoLC)fst01019597 _0 _9138684 |
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| 700 | 1 |
_aSharma, Arun Kumar, _eeditor literario |
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| 700 | 1 |
_aVarma, A. _q(Ajit), _d1939- _eeditor literario |
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| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=http://link.springer.com/10.1007/978-3-319-49197-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 988 | _aEBOOK, asignarmaterias, EBSPRINGER_2017C | ||
| 998 |
_b02/2018 _dz _e- _zSI |
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| 999 |
_c95877 _d95877 _x1 |
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