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008			161111s2016 gw | s |||| 0|eng d
020			_a9783319452951
024	7		_a10.1007/978-3-319-45295-1 _2doi
040			_aES-MaUEC
050		4	_aQP519.7 _b.J364 2016 EB
100	1		_aJanetzki, Sylvia _9101683 _0Local
245	1	0	_aElispot for Rookies (and Experts Too) _cby Sylvia Janetzki
260			_aCham _bSpringer International Publishing _c2016
300			_a1 recurso en línea (VIII, 93 páginas) _b39 ilustraciones, 32 ilustraciones en color
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
490	1		_aTechniques in Life Science and Biomedicine for the Non-Expert _x2367-1114
505	0		_aPreface -- Overview -- Historic Overview -- Principles of Elispot -- Modifications -- Simplifications -- Coating Step -- Detection Step -- Adaptations -- Detection of specific antibodies -- Detection of multiple analytes in one well -- Dual color Elispot -- Fluorospot -- Summary -- Important Assay Details -- Reagents and Materials -- Elispot plates -- Antibody choices -- Enzymatic conjugates and substrates -- Fluorescent dyes -- Culture media -- Blocking buffers -- Washing buffers -- Important laboratory supplies -- Pipettes -- Filters -- Other supplies -- Automated Elispot reader -- Sample preparation -- Overview -- Whole blood and PBMC -- Blood draw and anticoagulants -- PBMC isolation -- Time frame between blood draw and PBMC isolation -- Immune cells obtained from tissue -- Freezing of cells -- Thawing of cells -- Cell counting and viability -- Overnight resting -- Isolation of sub-populations of cells -- Expansion of cells -- Planning the experiment -- Controls -- Negative controls -- Positive controls -- External controls -- Plate layout -- Timing -- The Elispot assay -- Coating -- Blocking -- Effector cell preparation -- Stimulant preparation -- Peptides and peptide pools -- Proteins and lysate -- Antigen presenters -- Control stimulants -- Reference samples -- Plating out cells and stimulants -- Incubation -- Removal of cells -- Spot development -- Enumeration of spots -- Data analysis -- Quality control -- Reporting guidelines -- Troubleshooting -- References
520			_aThis book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author{u2019}s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGER
650		7	_aBioquímica _0comprobar BNE19974076456 _2embne _9138639
650		7	_aInmunología _0comprobar BNE19900958507 _2embne _9138330
650		7	_aCiencias biomédicas _0comprobar BNE19901047316 _2embne _9143729
830		0	_aTechniques in Life Science and Biomedicine for the Non-Expert _x2367-1114 _0http://id.loc.gov/authorities/names/n2016187336 _9134253
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-45295-1zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9783319452951
907			_a.b12981795 _b07-11-17 _c08-03-17
998			_am _a_alco _a_vill _b07-11-17 _cm _dz _eb _feng _ggw _h0
945			_aQP519.7 .J364 2016 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i1160363x _z06-04-17
999			_c88175 _d88175 _x1