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020 _a9783319300191
024 7 _a10.1007/978-3-319-30019-1
_2doi
040 _aES-MaUEC
050 4 _aQH506
_b.M53 2016 EB
082 0 4 _a616.9041
245 0 0 _aMicrofluidic Methods for Molecular Biology
_cedited by Chang Lu, Scott S. Verbridge
264 1 _aCham
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XII, 376 p.)
_b137 ilustraciones, 118 ilustraciones en color
336 _aTexto (visual)
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
505 0 _aMicrofluidic Platforms for Quantitative Biology Studies in Model Organisms -- Microfluidic Methods in Single Cell Biology -- Convective PCR Thermocycling with Smartphone-based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics -- Forensic Typing of Single Cells using Droplet Microfluidics -- Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA targets in Single Cells -- Microfluidic Multistage Integration for Analysis of Circulating Exosomes -- Microfluidic Single-Cell Functional Proteomics -- Microfluidics for DNA and Protein Analysis with Multiplex Microbead-based Assays -- Single Cell Phenotypic Screening in Inverse Metabolic Engineering -- Microfluidic Paper-based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers -- Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression -- Wire-guided Droplet Manipulation for Molecular Biology -- Mechanical and Electrical Principles for Separation of Rare Cells -- Microfluidics for High Throughput Cellular Isolation and Analysis in Biomedicine -- Microfluidics for Cell Culture -- Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations. .
520 3 _aThis book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics.
710 2 _aSpringerLink (Online service)
_0Local
_9106996
942 _2lcc
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988 _aEBOOK, EBSPRINGER
650 7 _aBiología molecular
_0comprobar BNE19900968373
_2embne
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650 7 _aPatología molecular
_9158317
_0comprobar BNE20010684648
_2embne
650 7 _aDiagnóstico molecular
_0comprobar BNE20012504786
_2embne
_9158916
700 1 _aLu, Chang
_d1976-
_0n 2010181614
_eeditor literario
_948594
700 1 _aVerbridge, Scott S.
_0n 2016187927
_eeditor literario
_948595
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-30019-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901 _ai9783319300191
907 _a.b12949310
_b11-11-17
_c21-11-16
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