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001			85295
003			ES-MaUEC
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008			160510s2016 gw | s |||| 0|eng d
020			_a9783319272160
040			_aES-MaUEC
050		4	_aTP248.65.P76 _bA383 2016
082	0	4	_a572.6
245	1	0	_aAdvanced Technologies for Protein Complex Production and Characterization _cedited by M Cristina Vega
260			_aCham _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XIII, 382 páginas) _b87 ilustraciones, 71 ilustraciones en color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
490	0		_aAdvances in Experimental Medicine and Biology _x0065-2598 _v896
505	0		_aProtein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants�potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. .
520	3		_aThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
710	2		_aSpringerLink (Online service) _0Local _9106996
942			_2lcc _cLE
988			_aEBOOK, asignarmaterias , EBSPRINGER
650	0	4	_9495993 _aCiencias de la vida
650		7	_aProteínas _0comprobar BNE19900977845 _2embne _9139861
700	1		_aVega, M. Cristina. _eeditor literario _998157 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-27216-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9783319272160
907			_a.b12945730 _b10-10-17 _c21-11-16
998			_am _a_alco _a_vill _b - - _cm _dz _e- _feng _ggw _h0
945			_aTP248.65.P76 A383 2016 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11589589 _z06-04-17
999			_c85295 _d85295 _x1