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008			150327s2015 ne | s |||| 0|eng d
020			_a9789401797160
024	7		_a10.1007/978-94-017-9716-0 _2doi
050		4	_aQM178 _b.H363 2015
245	1	0	_aHandbook of Vascular Biology Techniques _cedited by Mark Slevin, Garry McDowell.
260			_aDordrecht, Netherlands _bSpringer _c2015
300			_a1 recurso en línea (XII, 477 páginas) _b191 ilustraciones, 126 ilustraciones en color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
505	0		_aPreface -- Section 1 In vitro techniques -- Â{u9BA0} vitro angiogenesis assay - endothelial migration, proliferation, and tube formation -- Â{u5BA4}dothelial cell tube formation on basement membrane to study cancer neoangiogenesis -- Induction of hypoxia in vascular endothelial cell culture -- Evaluating In Vitro Angiogenesis using Live Cell Imaging -- Isolation of Endothelial Progenitor Cells (EPCs) -- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis -- Assessment of vascular function and contractility, ex vivo -- Measurement of Aβ uptake by cerebrovascular smooth muscle cells -- Â{uD961}asurement of Intracellular Ca2+ in Human Endothelial cells -- Section 2 In vivo and ex vivo manipulations -- Â{u5DA1}aluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion -- Â{u3C2F}nge implant model of inflammatory angiogenesis -- The chick embryo chorioallantoic membrane assay -- Dorsal air sac assay -- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals -- Hypoxia-induced retinal angiogenesis in adult zebrafish -- Angiogenesis in regenerating adult zebrafish tail fin -- Methods for studying developmental angiogenesis in zebrafish -- Isolation and Expansion of Brain Microvascular Endothelial Cells -- Section 3 Imaging and histological analysis -- Adipose angiogenesis -- Assessing tumor angiogenesis in histological samples -- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in theÂ{u096D}bryonic mouse skin and adult mouse cornea -- Computed Tomography Angiography: Fundamental techniques and data interpretation -- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation -- Single-Photon Emission Tomography of the brain in vascular pathology -- Section 4 Miscellaneous novel techniques in vascular biology -- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues -- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo -- Vascular Flow Modelling using Computational Fluid Dynamics -- Reverse Transcription Real-Time PCR Protocol for gene expression analyses -- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis -- Genomic microarray analysis -- Selection of appropriate housekeeping genes for quality control -- Endothelial transcriptomic analysis -- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers -- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells:Â{u0264}entification and Quantification -- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood -- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry.
520	3		_aA wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here. Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work. Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebro-vascular, cancer and dementia.
710	2		_aSpringerLink (Online service) _0Local _9106996
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGER, asignarmaterias_11febrero
650		7	_aLaboratorios _2embne _9140165
650		7	_aBiología _2embne _9138150
650	2	7	_aBiología _2embne _9138150
700	1		_aSlevin, Mark _eeditor literario _987961 _0Local
700	1		_aMcDowell, Garry _eeditor literario _994301 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-94-017-9716-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
907			_a.b12903061 _b10-10-17 _c18-01-16
998			_am _a_alco _a_vill _b - - _cm _dz _e- _feng _gne _h0
945			_aQM178 .H363 2015 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11572681 _z06-04-17
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