000			04935nam a22004215i 4500
001			81453
003			ES-MaUEC
005			20240611040137.0
007			cr nn 008mamaa
008			150919s2015 gw | s |||| 0|eng d
020			_a9783319199443
024	7		_a10.1007/978-3-319-19944-3 _2doi
050		4	_aQK673 _b.P53 2015 EB
082	0	4	_223
082	0	4	_223
245	1	0	_aPlant Microtechniques and Protocols _cedited by Edward Chee Tak Yeung, Claudio Stasolla, Michael John Sumner, Bing Quan Huang.
250			_a1st ed. 2015.
264		1	_aCham, Switzerland _bSpringer _c2015
300			_a1 recurso en línea (XIV, 576 páginas) _b88 ilustraciones, 68 ilustraciones en color
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
505	0		_aA Guide to the Study of Plant Structure with Emphasis on Living Specimens -- Chemical and Physical Fixation of Cells and Tissues: an Overview -- Paraffin and Polyester Waxes -- The Glycol Methacrylate Embedding Resins â€{u0525}chnovit 7100 and 8100 -- Epoxy Resins for Light and Transmission Electron Microscopy -- LR White Acrylic Resin -- High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples -- Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens -- Fluorescent Staining of Living Plant Cells -- Improved Methods for Clearing and Staining of Plant Samples -- Whole Mount Immunofluorescence Staining of Plant Cells and Tissues -- Protoplast Isolation and Staining -- Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving -- Abscisic Acid Immunostaining -- Plant Chromosome Preparations and Staining for Light Microscopic Studies -- Chromosome techniques and FISH -- Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2â€{uD925}oxyuridine (EdU) -- Staining Methods for Programmed Cell Death -- Laser Microdissection of Plant Tissues -- RNA In situ Hybridization -- Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy -- Collecting and Processing Wood Microcores for Monitoring Xylogenesis -- Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy -- Collection of Plant Remains from Archaeological Contexts -- Archaeological Wood Preparation -- Archaeopalynological Preparation Techniques -- Phytoliths: Preparation and Archaeological Extraction -- Starch Granules: Preparation and Archaeological Extraction -- Plant Collection, Identification, and Herbarium Procedures -- Index.
520			_aThis work gathers common plant microtechniquesÂ{u1BA4} updates their procedures using a simple and fully understandable approach.The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.This bookÂ{u1BA1}lyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume coversÂ{u4A25} more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled inÂ{u4A25} second sectionÂ{u4BE0}showcase the usefulness of various techniques based on different processing and staining methods.Â{u4A25} third sectionÂ{u8A67}hlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.
650		7	_9138490 _aAnatomía vegetal _2embne
700	1		_aHuang, Bing Quan _eeditor literario _993892 _0Local
700	1		_aStasolla, Claudio _eeditor literario _993890 _0Local
700	1		_aSumner, Michael John _eeditor literario _993891 _0Local _1http://viaf.org/viaf/103931862
700	1		_aYeung, Edward Chee Tak _eeditor literario _993889 _0Local
710	2		_aSpringerLink (Online service) _0Local _0http://id.loc.gov/authorities/names/no2005046756 _1http://viaf.org/viaf/148105729 _9106996
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-19944-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
907			_a.b12900436 _b10-10-17 _c18-01-16
942			_2lcc _cLE
945			_aSB123-123.5 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11570052 _z06-04-17
988			_aEBOOK, EBSPRINGER, GOBI_sep2018
988			_aEbook_one2one
998			_aSI _a_alco _a_vill _b10/2018 _cm _dz _ek _feng _ggw _h0
999			_c81453 _d81453 _x1