000			03023nam a22004215i 4500
999			_c81385 _d81385 _x1
001			81385
003			ES-MaUEC
005			20230207040327.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			150508s2015 gw | s |||| 0|eng d
020			_a9783319162591
024	7		_a10.1007/978-3-319-16259-1 _2doi
040			_bspa _cES-MaUEC _dES-MaUEC _aES-MaUEC
050		4	_aQK981.4 _b2015 EB
100	1		_aTill, Bradley J. _993771 _0Local
245	1	0	_aLow-Cost Methods for Molecular Characterization of Mutant Plants : _bTissue Desiccation, DNA Extraction and Mutation Discovery: Protocols _cby Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger.
264		1	_aCham, Switzerland _bSpringer _c2015
300			_a1 recurso en línea (X, 35 p.) _b9 ilustraciones, 3 ilustraciones en color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
505	0		_aIntroduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. Â{uE000}
506	0		_aOpen Access
520			_aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
988			_aEBOOK, EBSPRINGER
650		7	_aBiología molecular vegetal _2embne _9672192
650		7	_9676183 _aHibridación vegetal _2embne
700	1		_aJankowicz-Cieslak, Joanna _0Local _993772
700	1		_aHuynh, Owen A. _993773 _0Local
700	1		_aBeshir, Mayada M. _993774 _0Local
700	1		_aLaport, Robert G. _993775 _0Local
700	1		_aHofinger, Bernhard J. _993776 _0Local
710	2		_aSpringerLink (Online service) _0Local _9106996
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-16259-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2020 _dz _eIG _feng _ggw _h0