000			02832nam a22003255i 4500
999			_c81299 _d81299 _x1
001			81299
003			ES-MaUEC
005			20230202102831.0
007			cr nn 008mamaa
008			150105s2015 gw | s |||| 0|eng d
020			_a9783319123974
040			_aES-MaUEC
050		4	_aTP248.E5 _bR436 2015 EB
245	0	0	_aRecombinant Enzymes - From Basic Science to Commercialization _cedited by Azura Amid
260			_aCham, Switzerland _bSpringer _c2015
300			_a1 recurso en línea (X, 188 p.) 61 il.
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
520			_aThis edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product.Â{u04A5}aders will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host.Â{u0060}discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production.This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.Â{uE000}
650	0	7	_aEnzimas _9138640 _2embne
700	1		_aAmid, Azura _eeditor literario _993647 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-12397-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9783319123974
907			_a.b12898892 _b10-10-17 _c18-01-16
942			_2lcc _cLE
945			_aTP248.E5 R436 2015 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11568513 _z06-04-17
988			_aEBOOK, EBSPRINGER
998			_am _a_alco _a_vill _b10-03-17 _cm _dz _eo _feng _ggw _h0