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008 141028s2015 gw | s |||| 0|eng d
020 _a9783319101422
024 7 _a10.1007/978-3-319-10142-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK602
_b2015 EB
245 0 0 _aGenetic Transformation Systems in Fungi
_nVolume 1
_cedited by Marco A. van den Berg, Karunakaran Maruthachalam.
264 1 _aCham, Switzerland
_bSpringer
_c2015
300 _a1 recurso en línea (XV, 318 p.)
_b69 ilustraciones, 17 ilustraciones en color
336 _aTexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
490 1 _aFungal Biology
_x2198-7777
505 0 _aFungal Transformation: From Protoplasts to Targeted Recombination Systems -- Protoplast Transformation for Genome Manipulation in Fungi -- Trichoderma Transformation Methods -- Transformation of Mucor circinelloides f. lusitanicus Protoplasts -- Transformation of Saccharomyces cerevisiae: Spheroplast Method -- Electroporation Mediated DNA Transformation of Filamentous Fungi -- Chemical Transformation of Candida albicans -- Electroporation of Pichia pastoris -- Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) -- Biolistic Transformation for Delivering DNA into the Mitochondria -- Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants -- Use of the Biolistic Particle Delivery System to Transform Fungal Genomes -- Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment -- Agrobacterium tumefaciens-Mediated Transformation -- Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts -- Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum -- High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method -- Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism -- Transformation of Lithium Acetate-treated Neurospora crassa -- Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells -- Transformation of Fungi Using Shock Waves -- Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics -- Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle -- Recombination and Gene Targeting in Neurospora -- Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach -- REMI in Molecular Fungal Biology -- TALEN-Based Genome Editing in Yeast.
520 _aSeveral different transformation techniques have been developed over the years and readily shown to be decisive methodsÂ{u9BA0}fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examplesÂ{u5CE5}d in laboratories worldwide. Not only will this text provide a detailedÂ{u2863}kground onÂ{u1C30}lications inÂ{u9BA4}ustrial and pharmaceutical relevant microbes, but also theÂ{u9B70}ortance of fungal pathogensÂ{u9BA0}agricultural production (Phytophthora and Botrytis)Â{u1BA4} mammalian infection (Penicillium marneffei and Candida).Â{u796E}netic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungalÂ{u4CA1}its.
988 _aEBOOK, EBSPRINGER
650 7 _aHongos
_2embne
_9138683
700 1 _avan den Berg, Marco A
_eeditor literario
_993593
_0Local
700 1 _aMaruthachalam, Karunakaran
_eeditor literario
_993594
_0Local
710 2 _aSpringerLink (Online service)
_0Local
_9106996
830 0 _aFungal Biology
_x2198-7777
_9133157
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-319-10142-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2020
_dz
_eIG
_feng
_ggw
_h0