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001			76363
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007			cr nn 008mamaa
008			100301s2006 gw | s |||| 0|eng d
020			_a9783540327868
024	7		_a10.1007/3-540-32786-X _2doi
050		4	_aQP519.7 _bH658 2006
082	0	4	_a572
100	1		_aHoltzhauer, Martin _0Local _985394
245	1	0	_aBasic Methods for the Biochemical Lab _cby Martin Holtzhauer.
250			_aFirst English Edition.
260			_aBerlin, Heidelberg _bSpringer International Publishing _c2006
300			_a1 recurso en línea (XVIII, 251 p.) _b23 ilustraciones
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
490	1		_aSpringer Lab Manuals
505	0		_aQuantitative Methods -- Electrophoresis -- Chromatography -- Immunochemical Protocols -- Centrifugation -- Radioactive Labeling -- Buffers -- Tables -- Statistics and Data Analysis.
520			_aBasic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into the methods are mostly omitted. The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates), electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis), chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC), immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA), centrifugation (differential and density gradient centrifugation for cells and cellular fractions), radioactivity (labeling and counting), buffers (buffer properties and compositions). In additional chapters, tables compile a broad array of useful information, e.g. SI units conversion factor, detergent, protein and nucleotide data, and the basic principles of statistics and enzyme and receptor kinetics are reviewed. This first English-language edition of a successful German-language manual will be a valuable resource for students and experts in biochemistry, biotechnology and biomedical laboratories.
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGERrevisando
650		7	_aBiotecnología _0LocalV _2embne _9140931
650	2	7	_aBiotecnología _0LocalV _2embne _9140931
650	2	7	_aAnálisis clínicos _0LocalV _2embne _9138438
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/3-540-32786-X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
907			_a.b12819815 _b10-10-17 _c01-10-14
998			_am _a_alco _a_vill _b - - _cm _dz _e- _feng _ggw _h0
945			_aQP519.7 H658 2006 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11549038 _z06-04-17
999			_c76363 _d76363 _x1