000			03191nam a22003975i 4500
001			76311
003			ES-MaUEC
005			20230207040218.0
007			cr nn 008mamaa
008			100301s2006 gw s 000 0 eng d
020			_a9783540284895
024	7		_a10.1007/978-3-540-28489-5 _2doi
040			_aES-MaUEC
050		4	_aQH442.2 _b.M33 2006 EB
082	0	4	_a591.35
245	0	0	_aMammalian and Avian Transgenesis : _bNew Approaches _cedited by Shirley Pease, Carlos Lois
260			_aBerlin, Heidelberg _bSpringer International Publishing _c2006
300			_a1 recurso en línea (XX, 281 p.) _b49 ilustraciones, 12 en color
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
490	1		_aPrinciples and Practice
505	0		_aGeneration of Transgenic Animals Using Lentiviral Vectors -- Intracytoplasmic Sperm Injection (ICSI) in the Mouse -- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation -- Cloning The Laboratory Mouse by Nuclear Transfer -- Large Insert Transgenesis -- Regional and Temporal Control of Genetic Manipulation in the Mouse -- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes -- Nuclear Transfer in the Cow -- Production of Transgenic Pigs by DNA Microinjection -- Production of Transgenic Birds Using Lentiviral Vectors -- Ancillary Techniques
520	3		_aMammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGERrevisando
650		7	_aClonación _9153565 _0comprobar BNE19980042512 _2embne
650		7	_aGenética animal _0comprobar BNE19900960702 _2embne _9138513
700	1		_aPease, Shirley _eeditorliterario _985309 _0Local
700	1		_aLois, Carlos _eeditor literario _985310 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-540-28489-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9783540284895
907			_a.b12819293 _b10-10-17 _c01-10-14
998			_am _a_alco _a_vill _b15-07-17 _cm _dz _eu _feng _ggw _h0
945			_aQH442.2 .M33 2006 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11548514 _z06-04-17
999			_c76311 _d76311 _x1