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020 _a9783034808569
024 7 _a10.1007/978-3-0348-0856-9
_2doi
040 _aES-MaUEC
050 4 _aQP551
_b.F58 2014 EB
082 0 4 _a572.6
245 0 0 _aFluorescent methods for molecular motors
_cedited by Christopher P. Toseland, Natalia Fili
260 _aBasel
_bSpringer International Publishing
_c2014
300 _a1 recurso en línea (X, 298 p.)
_b88 ilustraciones, 49 ilustraciones en color
336 _aTexto (visual)
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
490 1 _aExperientia Supplementum
_x1023-294X
_v105
505 0 _aFluorescence and Labelling: How to choose and what to do -- Fluorescent biosensors: design and application to motor proteins -- Rapid Reaction Kinetic Techniques -- Fluorescence to study the ATPase mechanism of Motor Proteins -- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies -- Fluorescent methods to study transcription initiation and transition into elongation -- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo -- Fluorescence methods in the investigation of the DEAD-box helicase mechanism -- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins -- Fluorescence Tracking of Motor proteins in vitro -- Measuring Transport of Motor Cargos -- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET -- Using fluorescence to study actomyosin in yeasts
520 _aThis book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments
942 _2lcc
_cLE
988 _aEBOOK, EBSPRINGERrevisando
650 7 _aProteínas
_0comprobar BNE19900977845
_2embne
_9139861
650 7 _aProteínas
_xAnálisis
_9242917
_0comprobar BNE19971816444
_2embne
650 7 _aMicroscopía
_9138719
_0comprobar BNE19900963390
_2embne
700 1 _aToseland, Christopher P.
_eeditor literario
_985141
_0Local
700 1 _aFili, Natalia
_eeditor literario
_985142
_0Local
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-3-0348-0856-9
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901 _ai9783034808569
907 _a.b12818203
_b10-10-17
_c01-10-14
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