000			04037nam a22003375i 4500
001			76130
003			ES-MaUEC
005			20230207040210.0
007			cr nn 008mamaa
008			140514s2014 xxu| s |||| 0|eng d
020			_a9781493907182
040			_aES-MaUEC
050		4	_aRC280.B8 _bR877 2014 EB
100	1		_aRusso, Jose _d1942- _0no2012096047 _948399
245	1	0	_aTechniques and Methodological Approaches in Breast Cancer Research _cby Jose Russo, Irma H. Russo
260			_aNew York _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XVI, 287 p.) 192 il., 167 il. col.
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
520			_aThis book provides a detailed description of the most common techniques used in breast cancer research, with a special emphasis on the study of the normal breast as the basis for understanding the pathological processes in this organ. The authors have poured their experience in the field of breast cancer research into this book, and described the process of handling and interpreting the complex organ that is the human breast using original data and unique material collected over the years. Â{u4A25}y have established the concept of normality of the human breast and the morphological parameters to be used for distinguishing the normal from the diseased breast. The quality of the breast tissue obtained from autopsy material vs. core biopsies are carefully detailed and discussed, showing the morphological differences among the specimens according to their collection site. The use of laser capture microdissection and tissue microarray is described in detail and discussed with special references to the problems faced when the normal breast is under study. A chapter dedicated to the use of breast cancer cell lines is crafted around the importance of each cell line and its particularities for in vitro studies. In addition, the authors explore the new concept that cancer originates from two main cell types, basal and luminal cells . Particular attention is given to the study of the phenotypes commonly used for determining cell transformation, either spontaneously or by induced chemical agents. A companion chapter using in vivo models provides the adequate tools for those interested in studying the early events of cancer initiation, progression and metastasis. Special emphasis is given to the analysis of cells either by Flow cytometry and immuno-fluorescence techniques and the great potential of the use of in situ hybridization to study RNA transcripts. The book also features an original analysis of the putative markers of stem cells in breast cancer using immuno-cytochemical techniques, their interpretation and correlation with the prognosis of the disease. The last two chapters of the book are engaged in the utilization of RNA and DNA sequencing techniques and their values in understanding the physiological differences in the human breast, as well as the utilization of epigenetic methodologies in the understanding of the normal human breast. The authors have established the methodological basis for studying the human breast in its normal and pathological conditions providing a unique set of tools and valuable references for researchers pursuing a deeper understanding of the human breast
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGERrevisando
650		7	_aMamas _xCáncer _0comprobar BNE19901364206 _2embne _9182522
700	1		_aRusso, Irma H. _948398
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-1-4939-0718-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9781493907182
907			_a.b1281748x _b10-10-17 _c01-10-14
998			_am _a_alco _a_vill _b17-03-17 _cm _dz _ek _feng _gxxu _h0
945			_aRC280.B8 R877 2014 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11546700 _z06-04-17
999			_c76130 _d76130 _x1