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001			76125
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007			cr nn 008mamaa
008			140620s2014 xxua s 001 0 eng d
020			_a9781493906628
024	7		_a10.1007/978-1-4939-0662-8 _2doi
040			_aES-MaUEC
050		4	_aQP551 _b.M45 2014 EB
082	0	4	_a571.6
245	1	0	_aMembrane Proteins Production for Structural Analysis _cedited by Isabelle Mus-Veteau
264		1	_aNew York _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XXIII, 425 p.) _b108 ilustraciones, 72 ilustraciones en color
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
505	0		_a1 Membrane Proteins Production for Structural Analysis -- 2 Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies -- 3 Bacterial Expression and Stabilization of GPCRs -- 4 Membrane Protein Production in Escherichia Coli: Overview and Protocols -- 5 Lactococcus Lactis, Recent Developments in Functional Expression of Membrane Proteins -- 6 Over expression of Membrane Proteins in Saccharomyces Cerevisiae for Structural and Functional Studies: a Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid zFlippasey Complex Drs2p/Cdc50p -- 7 Amphipols: a General Introduction and Some Protocols -- 8 New Amphiphiles to Handle Membrane Proteins: zMénage à Troisy between Chemistry, Physical-Chemistry and Biochemistry -- 9 Building Model Membranes with Lipids and Proteins: Dangers and Challenges -- 10 Analytical Ultracentrifugation and Size Exclusion Chromatography Coupled to Light Scattering for the Characterization of Membrane Proteins in Solution -- 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins -- 12 Micelles, Bicelles, Amphipols, Nano discs, Liposomes or Intact Cells: the Hitchhiker's Guide to the Membrane Protein Study by NMR -- 13 Foundations of Bio molecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations and Free Energy Calculations of Membrane Proteins -- 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein.
520			_aThis volume reviews the latest development in production, stabilization and structural analysis techniques of membrane proteins. It contains 14 chapters exploring topics including the advances in heterologous expression systems, stabilization tools and structural methods that contributed to the growing number of recombinant integral membrane protein structures solved in the past few years. Each chapter was written by internationally renowned scientists in the field of membrane proteins structural characterization. Membrane proteins account for roughly 30 percent of all open reading frames in fully sequenced genomes. However, to date, atomic structures have so far been obtained for only 424 integral membrane proteins, with 100 new structures determined in the last two years. Only 10 percent of the unique integral membrane protein structures are derived from vertebrates. In general, integral membrane proteins are present in tissues at very low concentration, making production of recombinant proteins in heterologous systems suitable for large scale production a prerequisite for structural studies. Since the first atomic structures of recombinant mammalian integral membrane proteins published in 2005 (the calcium ATPase SERCA 1A and a voltage-dependent potatium channel), the structures of 37 recombinant mammalian integral membrane proteins, from which 20 belong to the G Protein Coupled Receptors family, have been solved.
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGERrevisando
650		7	_aProteínas _0comprobar BNE19900977845 _2embne _9139861
650		7	_aCitología _0LocalV _2embne _9139477
700	1		_aMus-Veteau, Isabelle _eeditorliterario _985012 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-1-4939-0662-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9781493906628
907			_a.b12817430 _b10-10-17 _c01-10-14
998			_am _a_alco _a_vill _b15-09-17 _cm _dz _ei _feng _gxxu _h0
945			_aQP551 .M45 2014 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11546657 _z06-04-17
999			_c76125 _d76125 _x1