| 000 | 03629nam a22003615i 4500 | ||
|---|---|---|---|
| 001 | 76056 | ||
| 003 | ES-MaUEC | ||
| 005 | 20230202101853.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 130917s2013 xxu| s |||| 0|eng d | ||
| 020 | _a9781461486664 | ||
| 040 | _aES-MaUEC | ||
| 050 | 4 |
_aQP551 _b.K568 2013 EB |
|
| 100 | 1 |
_aKinter, Michael _984895 _0Local |
|
| 245 | 1 | 0 |
_aApplication of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : _bA Replacement for Western Blot Analysis _cby Michael Kinter, Caroline S. Kinter. |
| 260 |
_aNew York _bSpringer International Publishing _c2013 |
||
| 300 | _a1 recurso en línea (XIII, 65 p.) 14 il., 11 il. col. | ||
| 336 |
_aTexto (visual) _btxt _2rdacontent |
||
| 337 |
_aelectrónico _bc _2rdamedia |
||
| 338 |
_arecurso electrónico _bcr _2rdacarrier |
||
| 490 | 1 |
_aSpringerBriefs in Systems Biology _x2193-4746 |
|
| 520 | _aÂ{u0060}key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. Â{u4A25} method that is most widely used isÂ{u4A25} Western blot analysis.Âרile Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations.Â{u026E} particular, the method is semi-quantitative with limited dynamic range.Âץstern blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment.ÂYw quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins.Â{u03EE}e of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Â{u396C}ected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites.The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages.Â{u036F}st importantly, it is fundamentally quantitative with a wide dynamic range.The output of the analysis is a numerical result that can range over several orders of magnitude.Â{u03F4}her advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures.Â{u01A9}nally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment.Â�This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins. | ||
| 942 |
_2lcc _cLE |
||
| 988 | _aEBOOK, EBSPRINGERrevisado | ||
| 650 | 7 |
_aProteínas _0comprobar BNE19900977845 _2embne _9139861 |
|
| 700 | 1 |
_aKinter, Caroline S. _984896 _0Local |
|
| 830 | 0 |
_aSpringerBriefs in Systems Biology _x2193-4746 _9133165 |
|
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-1-4614-8666-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 901 | _ai9781461486664 | ||
| 907 |
_a.b12816747 _b10-10-17 _c01-10-14 |
||
| 998 |
_am _a_alco _a_vill _b26-02-17 _cm _dz _eo _feng _gxxu _h0 |
||
| 945 |
_aQP551 .K568 2013 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11545963 _z06-04-17 |
||
| 999 |
_c76056 _d76056 _x1 |
||