000 03503nam a22003615i 4500
001 75862
003 ES-MaUEC
005 20230207040200.0
007 cr nn 008mamaa
008 130228s2013 xxu| s |||| 0|eng d
020 _a9781461443162
040 _aES-MaUEC
050 4 _aRM301.28
_b.B56 2013 EB
245 0 0 _aBiophysics for therapeutic protein development
_cedited by Linda O. Narhi
260 _aNew York
_bSpringer International Publishing
_c2013
300 _a1 recurso en línea (IX, 293 p.)
_b108 il. col
336 _aTexto (visual)
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
490 1 _aBiophysics for the Life Sciences
_v4
520 _aThe last few decades have seen the genesis of protein therapeutics, such that these large molecule based drugs comprise an increasingly larger part of the commercial market. The conformation (overall global fold or three-dimensional structure) of these molecules is important for maintaining biological activity, stability during long term storage, and can impact the safety profile and biological consequences of administration. For this reason at every stage of product discovery and development biophysical methods play a key analytical role in product development, as they are applied for the determination of protein higher order structure. In addition to helping define binding kinetics and other parameters important for target validation, they are the only techniques available to determine if a potential protein product is folded properly, and can maintain this active conformation during manufacturing, storage, and delivery. Thus the biophysical techniques play a key role during the development of protein therapeutics. This volume is organized to mimic the product lifecycle. The initial chapters describe the underlying theory, and strengths and weaknesses of the different techniques commonly used during therapeutic development. The majority of the chapters discuss the applications of these techniques, including case studies, across the product lifecycle from early discovery, where the focus is on identifying targets, and screening for potential drug product candidates, through expression and purification, large scale production, formulation development, lot-to-lot comparability studies, and commercial support including investigations. There is also a chapter from the perspective of the regulatory agencies. This book can be used to provide insight into this important application of biophysics for those who are planning a career in protein therapeutic development, and for those outside this area who are interested in understanding it better
942 _2lcc
_cLE
988 _aEBOOK, EBSPRINGERrevisado
650 7 _aFarmacología
_0comprobar BNE19900953030
_2embne
_9137893
650 7 _aBiofísica
_0comprobar BNE19900968115
_2embne
_9139082
650 7 _aProteínas
_0comprobar BNE19900977845
_2embne
_9139861
700 1 _aNarhi, Linda O.
_eeditor literario
_984552
_0Local
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-1-4614-4316-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901 _ai9781461443162
907 _a.b12814805
_b10-10-17
_c01-10-14
998 _am
_a_alco
_a_vill
_b14-03-17
_cm
_dz
_eb
_feng
_gxxu
_h0
945 _aRM301.28 .B56 2013 EB
_g1
_ieBOOK
_j0
_lmae
_o-
_pEUR0.00
_q-
_r-
_sb
_t15
_u0
_v0
_w0
_x0
_y.i11544181
_z06-04-17
999 _c75862
_d75862
_x1