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003			ES-MaUEC
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007			cr nn 008mamaa
008			100301s2006 ne | s |||| 0|eng d
020			_a9781402048968
040			_aES-MaUEC
050		4	_aQH441.5 _b.R485 2006 EB
082	0	4	_a571.6
245	0	0	_aReviews and Protocols in DT40 Research : _bSubcellular Biochemistry _cedited by Jean-Marie Buerstedde, Shunichi Takeda
260			_aDordrecht _bSpringer International Publishing _c2006
300			_a1 recurso en línea (XII, 478 p.)
336			_aTexto (visual) _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
520			_aResearch is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.
942			_2lcc _cLE
988			_aEBOOK, EBSPRINGERrevisado
650		7	_aCitogenética _0comprobar BNE19900974673 _2embne _9139622
700	1		_aBuerstedde, Jean-Marie _eeditor literario _984378 _0Local
700	1		_aTakeda, Shunichi _eeditor literario _984379 _0Local
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://link.springer.com/book/10.1007/978-1-4020-4896-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
901			_ai9781402048968
907			_a.b1281393x _b10-10-17 _c01-10-14
998			_am _a_alco _a_vill _b13-02-17 _cm _dz _ef _feng _gne _h0
945			_aQH441.5 .R485 2006 EB _g1 _ieBOOK _j0 _lmae _o- _pEUR0.00 _q- _r- _sb _t15 _u0 _v0 _w0 _x0 _y.i11543310 _z06-04-17
999			_c75775 _d75775 _x1