000 04013nam a22003615i 4500
999 _c399276
_d399276
001 399276
003 ES-MaUEC
005 20240518182517.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 230525s2023 si | o |||| 0|eng d
020 _a9789819913312
024 7 _a10.1007/978-981-99-1331-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRC269
_b2023 EB
100 1 _aAfify, Said M.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_9690275
245 1 0 _aMethods in Cancer Stem Cell Biology
_cby Said M Afify, Masaharu Seno
250 _a1st ed. 2023
264 1 _aSingapore
_bSpringer Nature Singapore
_c2023
300 _a1 recurso en línea
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
505 0 _a1. On the origin of cancer -- 2. Culture of Cells: Basic Principles -- 3. Stem cell culture from embryos -- 4. Reprogramming of normal cells -- 5. Maintenance of human Pluripotent Stem Cells -- 6. Identification of cancer stem cells by different molecular markers -- 7. Enrichment of Cancer Stem Cell from malignant tumor -- 8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line -- 9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells -- 10. Quick method to assess non-mutagenic carcinogens with iPS cells -- 11. Self-renewal potential of caner stem cells -- 12. Differentiation potential of cancer stem cells in vitro -- 13. Tumor Angiogenesis by cancer stem cells in vivo -- 14. Invasion and Metastatic potential of cancer stem cells in vitro -- 15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assay for Cancer Stem Cells: Tumorigenic assay in vitro -- 17. Tumorigenic potential of cancer stem cells in vivo -- 18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells -- 19. In-vitro evaluation of anti-cancer stem cell drugs -- 20. In vitro Tumoroid Model Using Cancer Stem Cells.
520 _aThis book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors' group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. .
988 _aSpringer_BiomedLife_2023
650 7 _2embne
_9146156
_aCélulas cancerosas
_xInvestigación
650 7 _2embne
_9160528
_aCélulas madre
_xInvestigación
700 1 _9690276
_aSeno, Masaharu
_eautor
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-99-1331-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2024
_dz
_eIG
_zSI