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020 _a9781071621851
024 7 _a10.1007/978-1-0716-2185-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.3
_b2022 EB
245 0 0 _aPlant Secondary Metabolism Engineering :
_bMethods and Protocols
_cedited by Arthur Germano Fett-Neto
250 _a2nd edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XII, 262 páginas)
_b65 ilustraciones, 40 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2469
505 0 _aExtraction and Analysis of Phenolic Compounds From Grape Berries -- Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli -- Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings -- A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja -- Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles -- Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential -- Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses -- Visualization of Suberization and Lignification in Sugarcane -- Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species -- Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS -- Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes -- Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging -- Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants -- Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa -- Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants -- Isolation of specialized plant cells by fluorescence-activated cell sorting -- X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts -- Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae -- Methods of mimosine extraction from Leucaena leucocephala (Lam.) de Wit leaves -- Microbial production, extraction, and quantitative analysis of isoprenoids.
520 _aThis second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field. .
988 _aSpringer_Protocols_2022
650 7 _2embne
_9140931
_aBiotecnología
776 0 8 _iPrinted edition:
_z9781071621844
776 0 8 _iPrinted edition:
_z9781071621868
776 0 8 _iPrinted edition:
_z9781071621875
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2185-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI