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008			220429s2022 xxu| o |||| 0|eng d
020			_a9781071622810
024	7		_a10.1007/978-1-0716-2281-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2022 EB
245	0	0	_aEpiblast Stem Cells : _bMethods and Protocols _cedited by Pierre Osteil
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 303 páginas) _b71 ilustraciones, 62 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2490
505	0		_aEstablishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence -- Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells -- Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution -- 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells -- Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells -- Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq -- A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data -- Complete Transcriptome Analysis by 5'-End Single-Cell RNA-Seq with Random Priming -- Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification -- Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells -- Generation of Gastruloids from Epiblast-Like Cells -- Definitive Endoderm from EpiSC Aggregates in Matrigel -- In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs) -- Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-Like Cells (hPGCLCs) In Vitro -- Differentiation of EpiLCs on Micro-Patterned Substrates Generated by Micro-Contact Printing -- Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence -- Modeling Epiblast Shape in Implanting Mammalian Embryos.
520			_aThis book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast.
988			_aSpringer_Protocols_2022
650		7	_2embne _9160528 _aCélulas madre
776	0	8	_iPrinted edition: _z9781071622803
776	0	8	_iPrinted edition: _z9781071622827
776	0	8	_iPrinted edition: _z9781071622834
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2281-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI