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020 _a9781071623411
024 7 _a10.1007/978-1-0716-2341-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 _aQH581.2
_b2022 EB
245 0 0 _aExtracellular Vesicles in Diagnosis and Therapy
_cedited by Maurizio Federico, Barbara Ridolfi
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XIV, 243 páginas)
_b45 ilustraciones, 31 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2504
505 0 _aAptasensors for Cancerous Exosome Detection -- Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor -- Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography -- Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis -- Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry -- Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles -- Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells -- Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery -- Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance -- Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay -- Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles -- Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum -- Extracellular Vesicles and their Use as Vehicles of Immunogens -- Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells -- Generation, Characterization, and Count of Fluorescent Extracellular Vesicles -- Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation -- Extracellular Vesicle Loading via pH-Gradient Modification.
520 _aThis detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9139477
_aCitología
776 0 8 _iPrinted edition:
_z9781071623404
776 0 8 _iPrinted edition:
_z9781071623428
776 0 8 _iPrinted edition:
_z9781071623435
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2341-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI