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008			220423s2022 xxua o |||| 0|eng d
020			_a9781071622537
024	7		_a10.1007/978-1-0716-2253-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981.4 _b2022 EB
245	0	0	_aPlant Gametogenesis : _bMethods and Protocols _cedited by Christophe Lambing
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 382 páginas) _b93 ilustraciones, 83 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2484
505	0		_aMeasurement of flower metabolite concentrations using Gas-Chromatography Mass Spectrometry and High-Performance Liquid Chromatography Mass Spectrometry -- Proteomic-, phosphoproteomic- and Acetylomic-based mass spectrometry to identify tissue-specific protein complexes and phosphorylation in plant gametogenesis -- Identifying small RNAs and analyzing their association with gene expression using isolated Arabidopsis male meiocytes -- Genotyping-by-sequencing to map meiotic crossovers in plants -- Interrogating global chromatin interaction network by high-throughput chromosome conformation capture (Hi-C) in plants -- Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat -- Super-resolution chromatin visualisation by combined fluorescence in situ hybridisation and Structured Illumination Microscopy in Solanum lycopersicum -- Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy -- Bimolecular Fluorescence Complementation to Test for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During Gametogenesis -- Quantifying meiotic crossover recombination in Arabidopsis lines expressing fluorescent reporters in seeds using SeedScoring pipeline for CellProfile -- Generation of mutants by combined treatment of physical and chemical mutagens in rice -- Generation of mutant plants by Gamma Ray exposure and development of low-cost TILLING population in chickpea (Cicer arietinum L.) -- Efficient generation of CRISPR/Cas9-based mutants supported by fluorescent seed selection in different Arabidopsis accessions -- Generation of deletion lines in Allohexaploid Bread Wheat -- Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato -- Protocols for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling -- Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis -- In vitro fertilization system using wheat gametes by electric fusion -- High-throughput fluorescent pollen tetrad analysis using DeepTetrad -- Barley (Hordeum vulgare) anther and meiocyte RNA Sequencing: Mapping Sequencing reads and downstream data analyses -- Profiling m6A RNA modifications in low amounts of plant cells using Maize Meiocytes -- Assembling Plant Genomes with Long-Read Sequencing -- Mapping-by-sequencing of point and insertional mutations with Easymap -- Characterization of the different levels of variation in 45S rRNA genes.
520			_aThis volume provides protocols and methods on techniques to study plant gametogenesis. Chapters are divided into four sections covering omics, cytological, molecular approaches, plant transformation, genome editing, bioinformatics, and data analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gametogenesis: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139640 _aGenética vegetal
776	0	8	_iPrinted edition: _z9781071622520
776	0	8	_iPrinted edition: _z9781071622544
776	0	8	_iPrinted edition: _z9781071622551
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2253-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI