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008			220422s2022 xxua o |||| 0|eng d
020			_a9781071622179
024	7		_a10.1007/978-1-0716-2217-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	0	_aVEGF Signaling : _bMethods and Protocols _cedited by Lorna R. Fiedler, Caroline Pellet-Many
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 353 páginas) _b62 ilustraciones, 55 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2475
505	0		_aOpposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies -- Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR -- Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells -- Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry -- Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays -- SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling -- Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells -- Co-Immunoprecipitation Assays -- Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling -- RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens -- Global MicroRNA Profiling of Vascular Endothelial Cells -- Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis -- Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro -- VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays -- Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy -- Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytometry -- Ex Vivo Mouse Aortic Ring Angiogenesis Assay -- Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability -- Preventing VEGF-Mediated Vascular Permeability by Experimentally Potentiating BBB Characteristics in Endothelial Cells -- The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis -- Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay -- Modulation of VEGFA Signaling during Heart Regeneration in Zebrafish -- Three-Dimensional Visualization of Blood and Lymphatic Vessels in the Adult Zebrafish Heart by Chemical Clearing -- Fluorescence-Activated Cell Sorting and Quantitative Real-Time PCR to Reveal VEGF Expressing Macrophage Populations in the Zebrafish Larvae -- Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model.
520			_aThis volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, VEGF Signaling: Methods and Protocols, Second Edition provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139861 _aProteínas
650		7	_2embne _9676130 _aEndotelio vascular
650		7	_2embne _9150702 _aTerapia genética
776	0	8	_iPrinted edition: _z9781071622162
776	0	8	_iPrinted edition: _z9781071622186
776	0	8	_iPrinted edition: _z9781071622193
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2217-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI