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008			220412s2022 xxua o |||| 0|eng d
020			_a9781071623374
024	7		_a10.1007/978-1-0716-2337-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH601 _b2022 EB
245	0	4	_aThe Nuclear Pore Complex : _bMethods and Protocols _cedited by Martin W. Goldberg
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 515 páginas) _b100 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2502
505	0		_aAffinity Isolation of Endogenous Saccharomyces cerevisiae Nuclear Pore Complexes -- Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes -- Nuclear Pore Complex Assembly using Xenopus Egg Extract -- Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans -- Hormone-inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases -- Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking -- Localising Total mRNA in Plant Cells -- Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues -- Analysis of Nucleoporin Function using Inducible Degron Techniques -- Monitoring of Chromatin Organization at the Nuclear Pore Complex, Inner Nuclear Membrane and Nuclear Interior in Live Cells by Fluorescence Ratiometric Imaging of Chromatin (FRIC) -- Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomic -- Protein Retargeting in Aspergillus nidulans to Study the Function of Nuclear Pore Complex Proteins -- Split-GFP Complementation to Study the Nuclear Membrane Proteome using Microscopy -- Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin a with Cargo Protein -- Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants -- Binding affinity measurement of Nuclear Export Signal peptides to their exporter CRM1 -- Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins -- Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope -- Crystallization of Nuclear Export Signals or Small Molecule Inhibitors Bound to Nuclear Exporter CRM1 -- Atomic Force Microscopy for Structural and Biophysical Investigations on Nuclear Pore Complexes -- Multivalent Interactions with Intrinsically Disordered Proteins Measured by Surface Plasmon Resonance -- Assembly and Use of a Microfluidic Device to Study Nuclear Mechanobiology during Confined Migration -- SPEED Microscopy: High-Speed Single-Molecule Tracking and Mapping of Nucleocytoplasmic Transport -- Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans -- Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts -- TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells -- Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast and Plants -- NPC Structure in Model Organisms: Transmission Electron Microscopy and Immuno-gold Labelling using High Pressure Freezing/Freeze Substitution of Yeast, Worms and Plants -- High Resolution Imaging and Analysis of Individual Nuclear Pore Complexes -- Live CLEM imaging of Tetrahymena to Analyze the Dynamic Behavior of the Nuclear Pore Complex -- Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy.
520			_aThis volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble transport factors involved in the transport process. Chapters detail structural biology methods, biophysical, transport factors , and a wide range of model organisms that have been used over the years. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Nuclear Pore Complex: Methods and Protocols: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9142266 _aMembranas celulares
776	0	8	_iPrinted edition: _z9781071623367
776	0	8	_iPrinted edition: _z9781071623381
776	0	8	_iPrinted edition: _z9781071623398
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2337-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _da _eb _zSI