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020 _a9781071619902
024 7 _a10.1007/978-1-0716-1990-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP363
_b2022 EB
245 0 0 _aAxonal Transport :
_bMethods and Protocols
_cedited by Alessio Vagnoni
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XV, 573 páginas)
_b145 ilustraciones, 126 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2431
505 0 _aThe Giant Axon of the Squid: A Simple System for Axonal Transport Studies -- Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica -- Live imaging of RNA Transport and Translation in Xenopus Retinal Axons -- Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves -- In vivo Imaging of Axonal Organelle Transport in the Mouse Brain -- Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI) -- Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins -- Imaging Diversity in Slow Axonal Transport -- Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses -- Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation -- Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons -- Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons -- Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons -- Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons -- High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells -- Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons -- In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons -- Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells -- Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves -- Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons -- Mitochondrial DNA Transport in Drosophila neurons -- Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System -- Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport -- High-resolution Live-Imaging of Axonal RNP granules in Drosophila Pupal Brain Explants -- Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans -- Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior -- Imaging Intracellular Trafficking in Neurons of C. elegans -- In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport -- In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport.
520 _aThis volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron. The chapters " Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9217236
_aNeuronas
_xFisiología
650 7 _2embne
_9158907
_aNeurociencias
776 0 8 _iPrinted edition:
_z9781071619896
776 0 8 _iPrinted edition:
_z9781071619919
776 0 8 _iPrinted edition:
_z9781071619926
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1990-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI