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008			220329s2022 xxua o |||| 0|eng d
020			_a9781071621325
024	7		_a10.1007/978-1-0716-2132-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2022 EB
245	0	0	_aPlasmodesmata : _bMethods and Protocols _cedited by Yoselin Benitez-Alfonso, Manfred Heinlein
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 470 páginas) _b90 ilustraciones, 87 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2457
505	0		_aPlasmodesmata Structural Components and Their Role in Signaling and Plant Development -- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses -- Plasmodesmata Ultrastructure Determination Using Electron Tomography -- Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata -- Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots -- Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities -- Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy -- Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy -- In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata -- Immunofluorescence Detection of Callose in Plant Tissue Sections -- Callose Detection and Quantification at Plasmodesmata in Bryophytes -- Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis -- Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons -- Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer -- Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy -- Using Steady-State Fluorescence Anisotropy to Study Protein Clustering -- Quantification of Cell-to-Cell Connectivity Using Particle Bombardment -- Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay -- Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay -- An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins -- Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP -- Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes -- Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability -- Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis -- Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling -- Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction -- A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue -- In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG -- Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM) -- More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight -- Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis.
520			_aThis fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9146302 _aCélulas vegetales
776	0	8	_iPrinted edition: _z9781071621318
776	0	8	_iPrinted edition: _z9781071621332
776	0	8	_iPrinted edition: _z9781071621349
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2132-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI