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008			220328s2022 xxua o |||| 0|eng d
020			_a9781071621608
024	7		_a10.1007/978-1-0716-2160-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .L9 _b2022 EB
245	0	0	_aNatural Killer (NK) Cells : _bMethods and Protocols _cedited by Noriko Shimasaki
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 330 páginas) _b50 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2463
505	0		_aIsolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria -- Purification of Primary Decidual Natural Killer Cells for Functional Analysis -- In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells -- Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells -- Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease -- Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq -- Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses -- Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells -- Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes -- Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis -- Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling -- Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer -- Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors -- Method to Study Adaptive NK Cells Following MCMV Infections -- Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains -- NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay -- Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions -- In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons -- CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells -- Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine -- Gene Transduction of Natural Killer Cells for Clinical Application.
520			_aThis detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.
988			_aSpringer_Protocols_2022
650		7	_2embne _9140675 _aLinfocitos
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781071621592
776	0	8	_iPrinted edition: _z9781071621615
776	0	8	_iPrinted edition: _z9781071621622
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2160-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI