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008			220324s2022 xxua o |||| 0|eng d
020			_a9781071618752
024	7		_a10.1007/978-1-0716-1875-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981.4 _b2022 EB
245	0	0	_aPlant Gene Silencing : _bMethods and Protocols _cedited by Kirankumar S. Mysore, Muthappa Senthil-Kumar
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 328 páginas) _b56 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2408
505	0		_aRecent Advances in Plant Gene Silencing Methods -- Strategies for Efficient RNAi Based Gene Silencing of Viral Genes For Disease Resistance In Plants -- Genome Editing and Designer Crops for the Future -- In Silico Methods for the identification of Viral-derived Small interfering RNAs (vsiRNAs) and their application in Plant genomics -- Barley stripe mosaic virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes In Wheat And Barley.-Virus-induced Gene Silencing in Wheat and Related Monocot Species -- Virus-Induced Gene Silencing in Sorghum using Brome mosaic virus -- RTBV-based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications -- Optimization of Tobacco rattle virus (TRV)-based Virus-Induced Gene Silencing (VIGS) in Tomato -- Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants -- VIGS-based Gene Silencing for Assessing Mineral Nutrient Acquisition -- High throughput Analysis of Gene Function Under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants -- A Method for Developing RNAi-derived Resistance in Cowpea Against Geminiviruses -- In vitro method for synthesis of large scale ds RNA molecule as a Novel Plant Protection Strategy -- Fine-tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs -- Trans-Kingdom RNA silencing in Plant-Fungal Disease Control -- An integrated bioinformatics and functional approach for miRNA validation -- Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants -- Multigene Transformation Through Cre-lox mediated Site-Specific Integration in Rice -- An Improvised Hairy Root Transformation Method for Efficient Gene Silencing in Roots and Nodules of Arachis hypogaea -- A method to reduce off-targets in CRISPR/Cas9 system in plants.
520			_aThis second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139640 _aGenética vegetal
776	0	8	_iPrinted edition: _z9781071618745
776	0	8	_iPrinted edition: _z9781071618769
776	0	8	_iPrinted edition: _z9781071618776
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1875-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI