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008			220323s2022 xxua o |||| 0|eng d
020			_a9781071621813
024	7		_a10.1007/978-1-0716-2181-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585 _b2022 EB
245	0	0	_aC. elegans : _bMethods and Applications _cedited by Gal Haspel, Anne C. Hart
250			_a3rd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 388 páginas) _b58 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2468
505	0		_aStarting a C. elegans Research Laboratory: Practical Advice -- The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans -- Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose -- Genetic Methods for Cellular Manipulation in C. elegans -- Observing and Quantifying Fluorescent Reporters -- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior -- Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior -- Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR) -- ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy -- A Fusion-PCR Method for Expressing Genetic Tools in C. elegans -- Approaches for CRISPR/Cas9 Genome Editing in C. elegans -- Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans -- Mutation Mapping and Identification by Whole-Genome Sequencing -- Lipid Extraction and Analysis -- Isolating Caenorhabditis elegans from the Natural Habitat -- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans -- Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser -- An Imaging System for Monitoring C. elegans Behavior and Aging -- Methods for Modulating and Measuring Neuromuscular Exertion in C. elegans -- Recording and Quantifying C. elegans Behavior -- Primer on Mathematical Modeling in C. elegans.
520			_aThis detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, Caenorhabditis elegans, a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, C. elegans: Methods and Applications, Third Edition offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms.
988			_aSpringer_Protocols_2022
650		7	_2embne _9301792 _aCitología _xTécnica
650		7	_2embne _9139475 _aCélulas
776	0	8	_iPrinted edition: _z9781071621806
776	0	8	_iPrinted edition: _z9781071621820
776	0	8	_iPrinted edition: _z9781071621837
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2181-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI