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008			220323s2022 xxua o |||| 0|eng d
020			_a9781071620557
024	7		_a10.1007/978-1-0716-2055-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .L42 _b2022 EB
245	0	0	_aGalectins : _bMethods and Protocols _cedited by Sean R. Stowell, Connie M. Arthur, Richard D. Cummings
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIX, 730 páginas) _b121 ilustraciones, 86 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2442
505	0		_aGalectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions -- Cloning, Expression and Purification of Galectins for In Vitro Studies -- Purification of Recombinant Galectins Using Affinity Chromatography Methods -- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation -- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method -- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction -- Evaluation of Galectin Binding by Surface Plasmon Resonance -- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein -- Examining Galectin Binding Specificity Using Glycan Microarrays -- Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study -- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis -- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries -- Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins -- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin -- What Happens if a Human Galectin Enters the Endoplasmic Reticulum? -- Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy -- Exploring the Galectin Network by Light and Fluorescence Microscopy -- Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners -- Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles -- Transcytosis of Galectin-3 in Mouse Intestine -- Evaluating the Role of Galectins in Clathrin Independent Endocytosis -- Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion -- Manipulating Galectin Expression in Zebrafish (Danio Rerio) -- Examining Galectin Gene Regulation by Reporter Assays -- Examining the Impact of Galectin-9 on Latent HIV Transcription -- Evaluation of the Role of Galectins in Parasite Immunity -- Evaluation of the Bactericidal Activity of Galectins -- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins -- Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin -- Analysis of Galectin-Binding Receptors on B Cells -- Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance -- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome -- Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay -- Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis -- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology -- Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle -- Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.
520			_aThis second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9666048 _aLectinas _vManuales de laboratorio
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071620540
776	0	8	_iPrinted edition: _z9781071620564
776	0	8	_iPrinted edition: _z9781071620571
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2055-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI