000			03865nam a2200397 i 4500
999			_c396607 _d396607
001			396607
003			ES-MaUEC
005			20230721084833.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			220226s2022 xxua o |||| 0|eng d
020			_a9781071620519
024	7		_a10.1007/978-1-0716-2051-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH205.2 _b2022 EB
245	0	0	_aFluorescent Microscopy _cedited by Bryan Heit
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 370 páginas) _b97 ilustraciones, 70 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2440
505	0		_a Fluorescence Microscopy: A Field Guide for Biologists -- Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy -- Optimizing Long-Term Live Cell Imaging -- Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy -- Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells -- Monitoring Cellular Responses to Infection with Fluorescent Biosensors -- Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF) -- Measurement of Minute Cellular Forces by Traction Force Microscopy -- Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues -- Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin -- Quantifiable Intravital Light Sheet Microscopy -- Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing -- Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos -- Super Resolution Radial Fluctuations (SRRF) Microscopy -- A Practical Guide for STED Imaging -- Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons -- Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy -- Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy -- Visualizing and Quantifying Data from Timelapse Imaging Experiments -- Automated Segmentation and Microscopy Image Analysis with Machine Learning.
520			_aThis volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9138719 _aMicroscopía
776	0	8	_iPrinted edition: _z9781071620502
776	0	8	_iPrinted edition: _z9781071620526
776	0	8	_iPrinted edition: _z9781071620533
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2051-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI