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008			220225s2022 xxua o |||| 0|eng d
020			_a9781071621448
024	7		_a10.1007/978-1-0716-2144-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB131 _b2022 EB
245	0	4	_aThe Inflammasome : _bMethods and Protocols _cedited by Ali A. Abdul-Sater
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 180 páginas) _b30 ilustraciones, 14 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2459
505	0		_aAssessment of ASC Oligomerization by Flow Cytometry -- In-vitro Assays to Study Inflammasome Activation in Primary Macrophages -- Measuring Non-Canonical Inflammasome Activation In Neutrophils -- Gasdermin D Cleavage Assay Following Inflammasome Activation -- Activation of the Non-Canonical Inflammasome In Mouse And Human Cells -- Measurement of Inflammasome-Induced Mitochondrial Dysfunction By Flow Cytometry -- Detection of ASC Oligomerization by Western Blotting -- Reconstitution System of NLRP3 Inflammasome in HEK293T Cells -- Intracellular Potassium Ions Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) -- NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots -- Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood -- Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation -- Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy -- In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 inflammasome Activation -- Inflammasome Activation in Gingival Epithelial Cells -- Imaging of Inflammasome Speck Formation in Living Cells.
520			_aThis volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Inflammasome: Methods and Protocols aims to provide protocols that will serve as tools for current and aspiring researchers interested in studying inflammasomes.
988			_aSpringer_Protocols_2022
650		7	_2embne _9153270 _aInflamación
650		7	_2embne _9138531 _aAntígenos
776	0	8	_iPrinted edition: _z9781071621431
776	0	8	_iPrinted edition: _z9781071621455
776	0	8	_iPrinted edition: _z9781071621462
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2144-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI