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008			220225s2022 xxua o |||| 0|eng d
020			_a9781071621288
024	7		_a10.1007/978-1-0716-2128-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC845 _b2022 EB
245	0	0	_aNon-Alcoholic Steatohepatitis : _bMethods and Protocols _cedited by Devanand Sarkar
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 361 páginas) _b79 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2455
505	0		_aHistopathological Diagnosis of Nonalcoholic Steatohepatitis (NASH) -- Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease -- Hydrodynamic Injection for Developing NASH Model -- Measurement of Hepatic Lipids -- Measurement of Fatty Acid Oxidation in Mammalian Cells -- Measurement of In Vivo VLDL and Chylomicron Secretion -- Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KC) -- Mouse Bone Marrow Cell Isolation and Macrophage Differentiation -- Purification and Isolation of Hepatic Stellate Cells -- Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes -- Culture of Mouse Liver Ductal Organoids -- Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived Liver Organoids -- Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver -- Analysis of Liver Responses to Non-Alcoholic Steatohepatitis by mRNA-Sequencing -- Single-Cell RNA-Sequencing in NASH -- Computational Pipeline for Next-Generation Sequencing (NGS) Studies in Genetics of NASH -- Metabolic Phenotyping in Mice with NASH using Indirect Calorimetry -- Analysis of Insulin Resistance in Nonalcoholic Steatohepatitis -- Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH) -- Ex Vivo Dual-Hit Method for Inflammasome Activation in Liver -- In Vivo Analysis of Necrosis and Ferroptosis in Nonalcoholic Steatohepatitis (NASH) -- Analysis of the Sphingolipidome in NAFLD -- Bile Acid Profiling in Mouse Biofluids and Tissues -- Preparation and Characterization of a Liver Targeted, Poly(amidoamine) Based, Gene Delivery System -- Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells -- Generation of Adenovirus for In Vitro and In Vivo Studies of Hepatocytes.
520			_aThis volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9169917 _aHígado _xEnfermedades
776	0	8	_iPrinted edition: _z9781071621271
776	0	8	_iPrinted edition: _z9781071621295
776	0	8	_iPrinted edition: _z9781071621301
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2128-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI