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008			220221s2022 xxua o |||| 0|eng d
020			_a9781071617915
024	7		_a10.1007/978-1-0716-1791-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.2 _b2022 EB
245	0	0	_aPlant Synthetic Biology : _bMethods and Protocols _cedited by Matias D. Zurbriggen
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 298 páginas) _b76 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2379
505	0		_aHeterologous production of plant terpenes in the photosynthetic bacterium Rhodobacter capsulatus -- Design of multiplexing CRISPR/Cas9 constructs for plant genome engineering using the GoldenBraid DNA assembly standard -- pSHDY - a new tool for genetic engineering of cyanobacteria -- Cas9-mediated targeted mutagenesis in plants -- Generation of stable, light-driven co-cultures of cyanobacteria with heterotrophic microbes -- Gene editing in green alga Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins -- Coexpression and reconstitution of enzymatic cascades in bacteria using UbiGate -- Homo-FRET imaging to study protein-protein interaction and complex formation in plants -- Plant X-tender toolbox for the assembly and expression of multiple transcriptional units in plants -- AGROBEST: a highly efficient Agrobacterium-mediated transient expression system in Arabidopsis seedlings -- Genetically-encoded biosensors for the quantitative analysis of auxin dynamics in plant cells -- Exploiting the Gal4/UAS system as plant orthogonal molecular toolbox to control reporter expression in Arabidopsis protoplasts -- Mathematical Modelling in Plant Synthetic Biology -- In vivo Epitope-tagging of Plant Mitochondria -- Engineering destabilizing N‑termini in plastids -- Trichome transcripts as efficiency control for Synthetic Biology and Molecular Farming.
520			_aThis volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9140931 _aBiotecnología
650		7	_2embne _9672737 _aBiología sintética
776	0	8	_iPrinted edition: _z9781071617908
776	0	8	_iPrinted edition: _z9781071617922
776	0	8	_iPrinted edition: _z9781071617939
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1791-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI