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008			220215s2022 xxua o |||| 0|eng d
020			_a9781071620878
024	7		_a10.1007/978-1-0716-2087-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.15 _b2022 EB
245	0	0	_aBrown Adipose Tissue : _bMethods and Protocols _cedited by David A. Guertin, Christian Wolfrum
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 309 páginas) _b72 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2448
505	0		_aBrown Adipose Tissue: A Short Historical Perspective -- Brown Fat Anatomy in Humans and Rodents -- Analysis of Thermogenesis Experiments with CalR -- A Clearing Method for Three-Dimensional Imaging of Adipose Tissue -- Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron -- Fluorescent and Luminescent Methods to Detect Lipolysis -- Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice -- Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes -- Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics -- Measurement of Futile Creatine Cycling Using Respirometry -- Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes -- Diet-Induced Thermogenesis: Principles and Pitfalls -- Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice -- Isolation and Characterization of Human Brown Adipocytes -- Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The 'Goldilocks Solution' -- Signaling Lipidomic Analysis of Thermogenic Adipocytes -- Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat -- In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond -- Analysis of Single Cell/Nucleus Transcriptome Data in Adipose Tissue.
520			_aThis detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would like to expand their toolkits. Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9667182 _aTejido adiposo
776	0	8	_iPrinted edition: _z9781071620861
776	0	8	_iPrinted edition: _z9781071620885
776	0	8	_iPrinted edition: _z9781071620892
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2087-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI