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008			220214s2022 xxua o |||| 0|eng d
020			_a9781071620755
024	7		_a10.1007/978-1-0716-2075-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR186.7 _b2022 EB
245	0	0	_aSingle-Domain Antibodies : _bMethods and Protocols _cedited by Greg Hussack, Kevin A. Henry
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 612 páginas) _b95 ilustraciones, 84 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2446
505	0		_a Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications -- Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development -- Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders -- Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders -- Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets -- A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications -- Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase -- Small Scale Secretory VHH Expression in Saccharomyces cerevisiae -- Production of Single-Domain Antibodies in Pichia pastoris -- Production of Designer VHH-Based Antibodies in Plant -- Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the ΔTm Shift -- Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing -- Engineering pH-Sensitive Single-Domain Antibodies -- Humanization of Camelid Single-Domain Antibodies -- Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues -- Introducing Cysteines into Nanobodies for Site-Specific Labelling -- Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles -- Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group -- Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells -- Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers for In Vivo Fluorescence Imaging and Image-Guided Surgery. -- Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments -- Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX -- Development of Glypican-2 Targeting Single-Domain Antibody CAR T Cells for Neuroblastoma -- Single-Domain Antibodies for Intracellular Toxin Neutralization -- Generation of Single-Domain Antibody-Based Recombinant Immunotoxins -- X-Ray Crystal Structure Analysis of VHH-Protein Antigen Complexes -- Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes -- Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants -- Peptide Tag-Specific Nanobodies for Studying Proteins in Live Cells -- Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology -- Optogenetic Activation of Intracellular Nanobodies. .
520			_aThis volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9143641 _aInmunoglobulinas
776	0	8	_iPrinted edition: _z9781071620748
776	0	8	_iPrinted edition: _z9781071620762
776	0	8	_iPrinted edition: _z9781071620779
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2075-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI