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008			220211s2022 xxua o |||| 0|eng d
020			_a9781071620359
024	7		_a10.1007/978-1-0716-2035-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH631 _b2022 EB
245	0	0	_aCell Polarity Signaling : _bMethods and Protocols _cedited by Chenbei Chang, Jianbo Wang
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 530 páginas) _b124 ilustraciones, 105 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2438
505	0		_aUse of Fluorescence Recovery after Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins -- FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs -- Live‐Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics -- Single-Cell Single Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates -- Biochemical Assays to Detect Activation of Small GTPases Rho, Rac, and Cdc42 during Morphogenesis -- Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos -- Regulation of Cell Polarity by Posttranslational Protein Palmitoylation -- Enrichment and Detection of Wnt Proteins from Cell Culture Media -- Using Live-Imaging to Examine Early Cardiac Development in Zebrafish -- Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm -- In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis -- Avian Embryos as a Model to Study Vascular Development -- BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice -- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics -- Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo -- Methods to Investigate Cell Polarity of Inner Ear -- Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice -- In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance -- Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance -- Dissection, Fixation, and Immunostaining of the Drosophila Midgut -- In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands -- Imaging Epidermal Cell Rearrangement in the C. elegans Embryo -- Methods for the Study of Apical Constriction during Ascidian Gastrulation -- Assays for Apical Constriction Using the Xenopus Model -- The Use of 3-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation -- Studying Cell Polarity Dynamics during Cancer Initiation Using Inducible 3D Organotypic Cultures -- Under-Agarose Chemotaxis and Migration Assays for Dictyostelium -- Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis -- Targeted Injections, Atomic Force Microscopy, and Hydrogels: A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo -- Quantitative Analysis of Directional Neural Crest Cell Migration.
520			_aThis volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity. The collection includes the usage of a wide variety of model systems and an extensive array of techniques, including genetic, imaging, biochemical, and biomechanical. Written for the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Cell Polarity Signaling: Methods and Protocols aims to enable researchers to delve into the stimulating field of cell polarity and contribute to our understanding of how coordinated control of protein stability, trafficking, membrane retention, post-translational modification, and dynamic organization leads to active regulation of cell polarity. Chapter 29 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9165668 _aFisiología celular
776	0	8	_iPrinted edition: _z9781071620342
776	0	8	_iPrinted edition: _z9781071620366
776	0	8	_iPrinted edition: _z9781071620373
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2035-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI