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008			220204s2022 xxua o |||| 0|eng d
020			_a9781071619209
024	7		_a10.1007/978-1-0716-1920-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP572 .S4 _b2022 EB
245	0	0	_aEstrogen Receptors : _bMethods and Protocols _cedited by Kathleen M. Eyster
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 427 páginas) _b81 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2418
505	0		_aAntibody Validation for Estrogen Receptor Beta -- Immunoblot Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30/GPER1 Activation -- Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver -- Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain -- Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils -- Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen -- In Situ Hybridization of Estrogen Receptors α and β in the Human Testis -- Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR -- PET Imaging of Estrogen Receptors Using 18F-Based Radioligands -- The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a Spatio-Temporal Dimension -- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model -- Assessing Estrogenic Activity of Classical Estrogen Receptor‑Binding Compounds -- An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta -- Polysome-Fractionation for Transcriptome-Wide Studies of mRNA Translation -- Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation -- Whole-Genome Genotyping Using DNA Microarrays for Population Genetics -- Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectrometry Approaches -- Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells -- Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and Its Co-Crystallization with a Target Peptide from Estrogen Receptor Alpha -- Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Targeted Protein Degradation of ERα -- Assessment of Metabolic Regulation by Estrogen Receptors -- RNA-Seq Experiment and Data Analysis.
520			_aThis fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Estrogen Receptors: Methods and Protocols, Second Edition serves as an ideal guide to researchers working to explore the vibrancy and excitement of the field of estrogen receptors and estrogen actions.
988			_aSpringer_Protocols_2022
650		7	_2embne _9675054 _aHormonas sexuales
650		7	_2embne _9139372 _aEndocrinología
776	0	8	_iPrinted edition: _z9781071619193
776	0	8	_iPrinted edition: _z9781071619216
776	0	8	_iPrinted edition: _z9781071619223
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1920-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI