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020 _a9781071621400
024 7 _a10.1007/978-1-0716-2140-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH599
_b2022 EB
245 0 0 _aChromatin :
_bMethods and Protocols
_cedited by Julia Horsfield, Judith Marsman
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XI, 379 páginas)
_b75 ilustraciones, 64 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2458
505 0 _aGenerating sequencing-based DNA methylation maps from low DNA input samples -- Data analysis of DNA methylation Epigenome-Wide Association Studies (EWAS); A guide to the principles of best practice -- Next-generation bisulfite sequencing for targeted DNA methylation analysis -- Editing of DNA Methylation Patterns Using CRISPR-Based Tools -- Nanopore sequencing and data analysis for base-resolution genome-wide 5-methylcytosine profiling -- Chromatin immunoprecipitation sequencing (ChIP-seq) protocol for small amounts of frozen biobanked cardiac tissue -- A robust protocol for investigating the cohesin complex by ChIP-sequencing -- Epi-Decoder: decoding the local proteome of a genomic locus by massive parallel chromatin immunoprecipitation combined with DNA-barcode sequencing -- A protocol for studying transcription factor dynamics using fast Single-Particle Tracking and Spot-On model-based analysis -- Characterization of mammalian regulatory complexes at single locus resolution using TINC -- Profiling protein-DNA interactions cell-type-specifically with Targeted DamID -- Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID -- The dCypher approach to interrogate chromatin reader activity against post-translational modification-defined histone peptides and nucleosomes -- High-resolution ATAC-seq analysis of frozen clinical tissues -- Single-molecule multikilobase-scale profiling of chromatin accessibility using m6A-SMAC-seq and m6A-CpG-GpC-SMAC-seq -- Circular chromosome conformation capture sequencing (4C-seq) in primary adherent cells -- Mammalian Micro-C-XL -- In situ HiC -- LncRNA-chromatin pull-down using biotin-conjugated DNA probes -- Super-resolution microscopy for visualization of physical contacts between chromosomes at nanoscale resolution.
520 _aThis volume provides cutting-edge techniques to further the study chromatin biology. Chapters include both novel and well-established methods for the analysis of DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data analysis pipelines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, provides details of the necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and describes step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromatin: Methods and Protocols aims to further the understanding of how modified DNA and associated proteins affect the transcriptional output of the genome. Chapter Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID [Chapter 12] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9144121
_aCromatina
776 0 8 _iPrinted edition:
_z9781071621394
776 0 8 _iPrinted edition:
_z9781071621417
776 0 8 _iPrinted edition:
_z9781071621424
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2140-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI