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008			220131s2022 xxua o |||| 0|eng d
020			_a9781071620595
024	7		_a10.1007/978-1-0716-2059-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP106.6 _b2022 EB
245	0	0	_aAngiogenesis : _bMethods and Protocols _cedited by Andrew V. Benest
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 429 páginas) _b122 ilustraciones, 108 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2441
505	0		_aThe Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo -- A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy -- Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling -- Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation -- Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina -- Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin -- Analyzing Lymphatic Vessel Patterning in Adult Tissue -- Transmission Electron Microscopy of Endothelium -- Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice -- Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts -- Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence -- An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery -- Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart -- Quantifying Lymphatic Vessel Density in Human Tissue Samples -- Quantifying Vascular Remodeling in the Mouse Spinal Cord -- Quantitative Methods to Assess Adipose Vasculature -- Quantification of Angiogenesis in Laser Choroidal Neovascularization -- A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue -- Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides -- Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells -- In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis -- Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates -- Isolation of Cardiac Endothelial Cells for Transcriptomic Analysis of the Zebrafish and Mouse Heart -- The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces -- Endothelial Cell Fibrin Gel Angiogenesis Bead Assay -- Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers -- Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications -- Finding and Verifying Enhancers for Endothelial-Expressed Genes -- Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching.
520			_aThis detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.
988			_aSpringer_Protocols_2022
650		7	_2embne _9164656 _aNeovascularización
650		7	_2embne _9670510 _aVasos sanguíneos
776	0	8	_iPrinted edition: _z9781071620588
776	0	8	_iPrinted edition: _z9781071620601
776	0	8	_iPrinted edition: _z9781071620618
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2059-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI