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008			220131s2022 xxua o |||| 0|eng d
020			_a9781071619162
024	7		_a10.1007/978-1-0716-1916-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP364 _b2022 EB
245	0	0	_aSynaptic Vesicles : _bMethods and Protocols _cedited by Jana Dahlmanns, Marc Dahlmanns
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 260 páginas) _b80 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2417
505	0		_aReal-time quantitative PCR and fluorescence in situ hybridization for subcellular localization of miRNAs in neurons -- Live-imaging of axonal cargoes in Drosophila brain explants using confocal microscopy -- Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents -- Quantitative analysis of presynaptic vesicle luminal pH in cultured neurons -- Investigation of synaptic vesicle proteins in rat brain tissue using real-time qPCR -- Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis -- A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting -- Synaptosomes and metamodulation of receptors -- A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations -- Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography -- Isolation of synaptic vesicles from mammalian brain -- Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis -- Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins -- Synaptic vesicle pool monitoring with synaptopH-Iuorin -- Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter -- Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures -- Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes -- Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors.
520			_aThis book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9147269 _aSinapsis
776	0	8	_iPrinted edition: _z9781071619155
776	0	8	_iPrinted edition: _z9781071619179
776	0	8	_iPrinted edition: _z9781071619186
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1916-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI