000			04162nam a2200397 i 4500
999			_c396577 _d396577
001			396577
003			ES-MaUEC
005			20230718074821.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			220105s2022 xxua o |||| 0|eng d
020			_a9781071617328
024	7		_a10.1007/978-1-0716-1732-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	4	_aThe Unfolded Protein Response : _bMethods and Protocols _cedited by Roberto Pérez-Torrado
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 332 páginas) _b64 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2378
505	0		_aMeasuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues -- Fluorescence-based biosensors for the detection of the unfolded protein response -- Indirect methods to measure unfolded proteins in living cells using fluorescent proteins -- A platform technology for monitoring the Unfolded Protein Response -- The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts -- High-throughput analysis of protein turnover with tandem fluorescent protein timers -- Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae -- Determination of protein translation during the activation of the UPR -- Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR -- Assays to study IRE1 activation and signaling -- Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis -- Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast -- Analyze mouse knockout models of UPR pathway elements -- In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS -- Detection of PERK signaling in the central nervous system -- Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans -- Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo -- Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana -- Structure-based drug discovery of IRE1 modulators -- Chemical screening to detect ER stress regulators.
520			_aThis volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781071617311
776	0	8	_iPrinted edition: _z9781071617335
776	0	8	_iPrinted edition: _z9781071617342
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1732-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI