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020 _a9781071620229
024 7 _a10.1007/978-1-0716-2022-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP461
_b2022 EB
245 0 0 _aDevelopmental, Physiological, and Functional Neurobiology of the Inner Ear
_cedited by Andrew K. Groves
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XIII, 398 páginas)
_b110 ilustraciones, 109 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aNeuromethods
_x1940-6045
_v176
505 0 _aPou4f3DTR Mice Enable Selective and Timed Ablation of Hair Cells in Post-Natal Mice -- Cochlear Explant Cultures: Creation and Application -- Immunohistochemistry and In Situ mRNA Detection using Inner Ear Vibratome Sections -- Genetic Manipulation of the Embryonic Chicken Inner Ear -- Molecular Tools to Study Regeneration of the Avian Cochlea and Utricle -- An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization -- A Manual Technique for Isolation and Single-Cell RNA Sequencing Analysis of Cochlear Hair Cells and Supporting Cells -- Ribosomal Pulldown Assays and Their Use to Analyze Gene Expression in Multiple Inner Ear Cell Types -- In Vivo Analysis of Hair Cell Sensory Organs in Zebrafish: From Morphology to Function -- Electrophysiological Recordings of Voltage Dependent and Mechanosensitive Currents in Sensory Hair Cell of the Auditory and Vestibular Organs of the Mouse -- Biophysical Recording from Adult Hair Cell -- Endocochlear Potential Measures, Local Drug Application, and Perilymph Sampling in the Mouse Inner Ear -- In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus -- Measurement of Human Cochlear and Auditory Nerve Potentials -- Strategies for Identification of Medial Olivocochlear Neurons for Patch-Clamp Studies of Synaptic Function using Electrical Stimulation and Optogenetics -- Auditory Brainstem Response (ABR) Measurements in Small Mammals -- Behavioral Models Loudness, Hyperacusis, and Sound Avoidance.
520 _aThis volume explores the latest techniques in inner ear development, analysis of its sensory cells, and characterization and manipulation of the central auditory and vestibular pathways. The chapters in this book cover topics such as dissection and imaging of the cochlea; behavioral evaluation of animal models of diseases like tinnitus; hair cell function and regeneration; and recent advances in sequencing technology. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Developmental, Physiological, and Function Neurobiology of the Inner Ear is a valuable resource for scientists and researchers interested in learning more about this developing field. .
988 _aSpringer_Protocols_2022
650 7 _2embne
_9138188
_aAudición
776 0 8 _iPrinted edition:
_z9781071620212
776 0 8 _iPrinted edition:
_z9781071620236
776 0 8 _iPrinted edition:
_z9781071620243
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2022-9
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI