000 03850nam a2200409 i 4500
999 _c396574
_d396574
001 396574
003 ES-MaUEC
005 20230718072703.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 211222s2022 xxua o |||| 0|eng d
020 _a9781071621486
024 7 _a10.1007/978-1-0716-2148-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP551
_b2022 EB
245 0 0 _aGlycan Microarrays :
_bMethods and Protocols
_cedited by Michelle Kilcoyne, Jared Q. Gerlach
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XI, 253 páginas)
_b72 ilustraciones, 65 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2460
505 0 _aNeoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins -- Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system -- Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies -- Nanocube-based fluidic glycan array -- General strategies for glycan microarray data processing and analysis -- Calculating half maximal inhibitory concentration (IC50) values from glycomics microarray data using GraphPad Prism -- Synthetic plant glycan microarrays as tools for plant biology -- Mucin purification and printing natural mucin microarrays -- Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins -- Tissue glycome mapping: Lectin microarray-based differential glycomic analysis of formalin-fixed paraffin-embedded tissue sections -- Detection of autoantibodies using combinatorial glycolipid microarrays -- Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development -- Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system -- Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation -- Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays.
520 _aThis volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. 'Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins, various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9139861
_aProteínas
650 7 _2embne
_9138639
_aBioquímica
776 0 8 _iPrinted edition:
_z9781071621479
776 0 8 _iPrinted edition:
_z9781071621493
776 0 8 _iPrinted edition:
_z9781071621509
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2148-6
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI