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008			211225s2022 xxua o |||| 0|eng d
020			_a9781071620717
024	7		_a10.1007/978-1-0716-2071-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC268.4 _b2022 EB
245	0	0	_aAutophagy and Cancer : _bMethods and Protocols _cedited by Helin Norberg, Erik Norberg
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 353 páginas) _b97 ilustraciones, 84 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2445
505	0		_aA balance between autophagy and other cell death modalities in cancer -- Isolation of Autophagy competent Lysosomes from Cancer cells by Differential Large-scale Multi-layered Density Gradient Centrifugations -- CRISPR-Cas9 Gene Editing to create Isoform specific LAMP-2A Knockout in Human Cancer Cells -- Modified LC3 dot quantification method -- A quantitative flow cytometry-based method for autophagy detection across the cell cycle -- A dual HiBiT-GFP-LC3 lentiviral reporter for autophagy flux assessment -- Measuring autophagic cargo flux with Keima-based probes -- Detection of nuclear biomarkers for chromosomal instability -- Analysis of autophagic vesicles in mitotic cells -- Approaches and protocols to analyze autophagy and its role in death of apoptosis-resistant senescent tumor cells -- Concepts and design of introducing synthetic microRNAs into mammalian cells -- Studying autophagy using a TMT-based quantitative proteomics approach -- Immunofluorescence-based measurement of autophagosome formation during mitophagy -- Induction and detection of mitophagy -- Analysis of pancreatic acinar protein solubility in autophagy-deficient mice -- Studying autophagy in vivo in the mammary gland and in xenograft samples -- A method to Identify Potential Prognostic Markers Across Distinct Tumor Types -- A method for Co-Expression Analysis -- Methodological approaches for assessing metabolomic changes in glioblastomas -MS -- Extraction of Metabolites from Cancer Cells -- Measuring glucose consumption and gluconeogenesis in 3D human tissue cultures with nanoliter input volumes. .
520			_aThis volume includes comprehensive methods and protocols on autophagic pathways in cancer disorders. Chapters cover basic principles of autophagy methodology in cancer cells, translational approaches, systematic computational analyses of TCGA (The Cancer Genome Atlas) cohorts, CCLE (The Cancer Cell Line Encyclopedia) analysis, CRISPR genetics, metabolomics, biochemistry, and immunohistochemistry methodologies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Autophagy and Cancer: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9273952 _aCáncer _xAspectos genéticos
776	0	8	_iPrinted edition: _z9781071620700
776	0	8	_iPrinted edition: _z9781071620724
776	0	8	_iPrinted edition: _z9781071620731
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2071-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI